Kinetics of insulin aggregation in aqueous solutions upon agitation in the presence of hydrophobic surfaces.

V Sluzky, JA Tamada, AM Klibanov… - Proceedings of the …, 1991 - National Acad Sciences
The stability of protein-based pharmaceuticals (eg, insulin) is important for their production,
storage, and delivery. To gain an understanding of insulin's aggregation mechanism in …

Resistive-pulse studies of proteins and protein/antibody complexes using a conical nanotube sensor

LT Sexton, LP Horne, SA Sherrill… - Journal of the …, 2007 - ACS Publications
There is increasing interest in using nanopores in synthetic membranes as resistive-pulse
sensors for molecular and macromolecule analytes. In general, this method entails …

Mechanism of insulin aggregation and stabilization in agitated aqueous solutions

V Sluzky, AM Klibanov, R Langer - Biotechnology and …, 1992 - Wiley Online Library
Undesirable aggregation of aqueous insulin solutions remains a serious obstacle in the
development of alternative methods of diabetes therapy. We investigated the fundamental …

Rapid detection of Hendra virus antibodies: an integrated device with nanoparticle assay and chaotic micromixing

K Petkovic, G Metcalfe, H Chen, Y Gao, M Best… - Lab on a Chip, 2017 - pubs.rsc.org
Current diagnosis of infectious diseases such as Hendra virus (HeV) relies mostly on
laboratory-based tests. There is an urgent demand for rapid diagnosis technology to detect …

Antigen-induced oligomerization of the B cell receptor is an early target of FcγRIIB inhibition

W Liu, H Won Sohn, P Tolar, T Meckel… - The Journal of …, 2010 - journals.aai.org
The FcγRIIB is a potent inhibitory coreceptor that blocks BCR signaling in response to
immune complexes and, as such, plays a decisive role in regulating Ab responses. The …

Quantum dot/carrier–protein/haptens conjugate as a detection nanobioprobe for FRET-based immunoassay of small analytes with all-fiber microfluidic biosensing …

F Long, C Gu, AZ Gu, H Shi - Analytical chemistry, 2012 - ACS Publications
This study demonstrates the use of carrier-protein/haptens conjugate (eg, BSA/2, 4-
dichlorophenoxyacetic acid, 2, 4-D-BSA) for biological modification of quantum dots (QDs) …

Protein A immunoaffinity hollow fiber membranes for immunoglobulin G purification: experimental characterization

C Charcosset, Z Su, S Karoor, G Daun… - Biotechnology and …, 1995 - Wiley Online Library
Immunoaffinity adsorption is increasingly used for protein purification and medical
applications. Synthetic membranes have advantages as support matrices in comparison to …

Evaluation of nanoparticle tracking analysis for the detection of rod-shaped particles and protein aggregates

BM Hoover, RM Murphy - Journal of pharmaceutical sciences, 2020 - Elsevier
Nanoparticle tracking analysis (NTA) is an important technique for measuring hydrodynamic
size of globular biological particles including liposomes and viruses. Less attention has …

Size and structure of antigen-antibody complexes. Electron microscopy and light scattering studies

RM Murphy, H Slayter, P Schurtenberger… - Biophysical journal, 1988 - cell.com
Size parameters of model antigen-antibody (Ag-Ab) complexes formed by the interaction of
bovine serum albumin (BSA) and pairs of monoclonal anti-BSA antibodies (mAb) were …

An analysis of transport resistances in the operation of BIAcore™; implications for kinetic studies of biospecific interactions

ML Yarmush, DB Patankar, DM Yarmush - Molecular Immunology, 1996 - Elsevier
The mass-transfer processes that affect kinetic measurements of biospecific interactions
between one species in a flowing solution and another species immobilized in a thin …