The molecular virology of coronaviruses

E Hartenian, D Nandakumar, A Lari, M Ly… - Journal of Biological …, 2020 - ASBMB
Few human pathogens have been the focus of as much concentrated worldwide attention as
severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of COVID-19. Its …

Nsp3 of coronaviruses: Structures and functions of a large multi-domain protein

J Lei, Y Kusov, R Hilgenfeld - Antiviral research, 2018 - Elsevier
The multi-domain non-structural protein 3 (Nsp3) is the largest protein encoded by the
coronavirus (CoV) genome, with an average molecular mass of about 200 kD. Nsp3 is an …

The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein

S Lu, Q Ye, D Singh, Y Cao, JK Diedrich… - Nature …, 2021 - nature.com
The multifunctional nucleocapsid (N) protein in SARS-CoV-2 binds the~ 30 kb viral RNA
genome to aid its packaging into the 80–90 nm membrane-enveloped virion. The N protein …

[HTML][HTML] Crystal structure of SARS-CoV-2 nucleocapsid protein RNA binding domain reveals potential unique drug targeting sites

S Kang, M Yang, Z Hong, L Zhang, Z Huang… - … Pharmaceutica Sinica B, 2020 - Elsevier
The outbreak of coronavirus disease (COVID-19) caused by SARS-CoV-2 virus continually
lead to worldwide human infections and deaths. Currently, there is no specific viral protein …

[HTML][HTML] The SARS-CoV-2 nucleocapsid protein is dynamic, disordered, and phase separates with RNA

J Cubuk, JJ Alston, JJ Incicco, S Singh… - Nature …, 2021 - nature.com
Abstract The SARS-CoV-2 nucleocapsid (N) protein is an abundant RNA-binding protein
critical for viral genome packaging, yet the molecular details that underlie this process are …

Phosphoregulation of phase separation by the SARS-CoV-2 N protein suggests a biophysical basis for its dual functions

CR Carlson, JB Asfaha, CM Ghent, CJ Howard… - Molecular cell, 2020 - cell.com
The nucleocapsid (N) protein of coronaviruses serves two major functions: compaction of the
RNA genome in the virion and regulation of viral gene transcription. It is not clear how the N …

Genomic RNA elements drive phase separation of the SARS-CoV-2 nucleocapsid

C Iserman, CA Roden, MA Boerneke, RSG Sealfon… - Molecular cell, 2020 - cell.com
We report that the SARS-CoV-2 nucleocapsid protein (N-protein) undergoes liquid-liquid
phase separation (LLPS) with viral RNA. N-protein condenses with specific RNA genomic …

SARS‐CoV‐2 nucleocapsid protein phase‐separates with RNA and with human hnRNPs

TM Perdikari, AC Murthy, VH Ryan, S Watters… - The EMBO …, 2020 - embopress.org
Tightly packed complexes of nucleocapsid protein and genomic RNA form the core of
viruses and assemble within viral factories, dynamic compartments formed within the host …

Structural basis of RNA recognition by the SARS-CoV-2 nucleocapsid phosphoprotein

DC Dinesh, D Chalupska, J Silhan, E Koutna… - PLoS …, 2020 - journals.plos.org
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of
the coronavirus disease 2019 (COVID-19). SARS-CoV-2 is a single-stranded positive-sense …

Secondary structure determination of conserved SARS-CoV-2 RNA elements by NMR spectroscopy

A Wacker, JE Weigand, SR Akabayov… - Nucleic acids …, 2020 - academic.oup.com
The current pandemic situation caused by the Betacoronavirus SARS-CoV-2 (SCoV2)
highlights the need for coordinated research to combat COVID-19. A particularly important …