Measuring DNA modifications with the comet assay: a compendium of protocols

A Collins, P Møller, G Gajski, S Vodenková… - Nature protocols, 2023 - nature.com
The comet assay is a versatile method to detect nuclear DNA damage in individual
eukaryotic cells, from yeast to human. The types of damage detected encompass DNA …

Chromium toxicity and tolerance in plants

HP Singh, P Mahajan, S Kaur, DR Batish… - Environmental Chemistry …, 2013 - Springer
Chromium (Cr) is the second most common metal contaminant in ground water, soil, and
sediments due to its wide industrial application, hence posing a serious environmental …

Cadmium and plant development: An agony from seed to seed

M Huybrechts, A Cuypers, J Deckers, V Iven… - International journal of …, 2019 - mdpi.com
Anthropogenic pollution of agricultural soils with cadmium (Cd) should receive adequate
attention as Cd accumulation in crops endangers human health. When Cd is present in the …

Complexities of chromium carcinogenesis: role of cellular response, repair and recovery mechanisms

TJ O'Brien, S Ceryak, SR Patierno - Mutation Research/Fundamental and …, 2003 - Elsevier
Certain hexavalent chromium (Cr (VI))-containing compounds are recognized occupational
human lung carcinogens and may pose an environmental health risk. The carcinogenicity of …

DNA strand breakage in aquatic organisms and the potential value of the comet assay in environmental monitoring

CL Mitchelmore, JK Chipman - Mutation Research/Fundamental and …, 1998 - Elsevier
This review considers the potential for DNA strand breaks, particularly as measured by the
comet assay, to act as a biomarker of genetic toxicity in fish and other aquatic species. The …

Single cell gel electrophoresis assay: methodology and applications

E Rojas, MC Lopez, M Valverde - Journal of Chromatography B …, 1999 - Elsevier
The single cell gel electrophoresis or Comet assay is a sensitive, reliable, and rapid method
for DNA double-and single-strand breaks, alkali-labile sites and delayed repair site …

Cadmium toxicity in plants

E Andresen, H Küpper - Cadmium: from toxicity to essentiality, 2013 - Springer
Cadmium is an important pollutant in the environment, toxic to most organisms and a
potential threat to human health: Crops and other plants take up Cd from the soil or water …

Trivalent chromium: assessing the genotoxic risk of an essential trace element and widely used human and animal nutritional supplement

DA Eastmond, JT MacGregor… - Critical reviews in …, 2008 - Taylor & Francis
Trivalent chromium [Cr (III)] is recognized as an essential nutrient, and is widely used as a
nutritional supplement for humans and animals. Recent reports of the induction of genetic …

Comet assay in genetic ecotoxicology: a review

S Cotelle, JF Férard - Environmental and molecular …, 1999 - Wiley Online Library
The Comet assay, also called the single cell gel electrophoresis (SCGE) assay or microgel
electrophoresis (MGE) assay, primarily measures DNA strand breakage in single cells …

DNA damage and repair in individual cells: applications of the comet assay in radiobiology

PL Olive - International journal of radiation biology, 1999 - Taylor & Francis
The comet assay is a single-cell gel electrophoresis method that can measure a variety of
types of DNA damage, and repair of damage, in individual cells. It is now in widespread use …