Tissue and organ biology are very challenging to study in mammals, and progress can be
hindered, particularly in humans, by sample accessibility and ethical concerns. However …

We have greatly advanced our ability to grow a diverse range of tissue-derived and
pluripotent stem cell–derived gastrointestinal (GI) tissues in vitro. These systems, broadly …

Stem-cell-derived epithelial organoids are routinely used for the biological and biomedical
modelling of tissues. However, the complexity, lack of standardization and quality control of …

The lung epithelium is derived from the endodermal germ layer, which undergoes a complex
series of endoderm–mesoderm-mediated signaling events to generate the final arborized …

We report the generation of human ESC-derived, expandable hepatic organoids (hEHOs)
using our newly established method with wholly defined (serum-free, feeder free) media …

The study of biliary disease has been constrained by a lack of primary human
cholangiocytes. Here we present an efficient, serum-free protocol for directed differentiation …

Regeneration and homeostasis in the adult intestinal epithelium is driven by proliferative
resident stem cells, whose functional properties during organismal development are largely …

Tractable and accurate disease models are essential for understanding disease
pathogenesis and for developing new therapeutics. As stem cells are capable of self …

The intestinal mucosa forms the first line of defense against infections mediated by enteric
pathogens such as salmonellae. Here we exploited intestinal “organoids”(iHOs) generated …

Tissues and organs provide the structural and biochemical landscapes upon which
microbial pathogens and commensals function to regulate health and disease. While flat two …