The first blood transfusions in humans were xenotransfusions, carried out by Jean‐Baptiste
Denis beginning in 1667. Richard Lower, Matthäus Purmann and Georges Mercklin also …

An alternative to human red blood cells (RBCs) for clinical transfusion would be
advantageous, particularly in situations of massive acute blood loss (where availability and …

PEGylation induced changes in molecular volume and solution properties of HbA have been
implicated as potential modulators of its vasoconstrictive activity. However, our recent …

Our recent studies on PEG–Hb [poly (ethylene glycol)–Hb] conjugates generated by
thiolation-mediated maleimide-chemistry based PEGylation demonstrated that the …

The influence of intramolecular cross-links on the molecular, structural and functional
properties of PEGylated {PEG [poly (ethylene glycol)]-conjugated} haemoglobin has been …

The engineering of animals for recombinant protein production has gone beyond the stage
of identifying proper regulatory sequences. Efforts are now spent on the generation of …

Bis (maleidophenyl)-PEG2000 (Bis-Mal-PEG2000), a new bifunctional protein cross-linker
targeted to sulfhydryl groups, introduces intra-tetrameric cross-links into oxy-HbA in nearly …

Fig 1. Engineering the mammary gland of a transgenic animal bioreactor. Human plasma
proteins are targeted for expression to specific organs like the mammary gland of transgenic …

Hemoglobin (Hb) is a tetrameric protein (mol wt= 64,500) and is the major protein
component of red blood cells (RBCs). In normal human erythrocytes, HbA composes about …

The regulatory elements of genes used to target the tissue-specific expression of
heterologous human proteins have been studied in vitro and in transgenic mice. Hybrid …