Gene editing that makes target gene modification in the genome by deletion or addition has
revolutionized the era of biomedicine. Clustered regularly interspaced short palindromic …

The innovation of biotechnologies has allowed the accumulation of omics data at an
alarming rate, thus introducing the era of 'big data'. Extracting inherent valuable knowledge …

CRISPR gene editing holds great promise to modify DNA sequences in somatic cells to treat
disease. However, standard computational and biochemical methods to predict off-target …

A major challenge for effective application of CRISPR systems is to accurately predict the
single guide RNA (sgRNA) on-target knockout efficacy and off-target profile, which would …

CRISPR-Cas genome editing induces targeted DNA damage but can also affect off-target
sites. Current off-target discovery methods work using purified DNA or specific cellular …

The past decade has witnessed a rapid evolution in identifying more versatile clustered
regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) …

In the field of cancer genomics, the broad availability of genetic information offered by next-
generation sequencing technologies and rapid growth in biomedical publication has led to …

The adaptive immunity system in bacteria and archaea, Clustered Regularly Interspaced
Short Palindromic Repeats, CRISPR-associate (CRISPR/Cas), has been adapted as a …

Abstract The Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/CRISPR-
associated (Cas) system has emerged as the main technology for gene editing. Successful …

Metabolic engineering encompasses several widely-used strategies, which currently hold a
high seat in the field of biotechnology when its potential is manifesting through a plethora of …