Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …

CRISPR-Cas technologies have the potential to revolutionize genetic medicine. However,
work is still needed to make this technology clinically efficient for gene correction. A barrier …

DNA repair in mammalian cells involves the coordinated action of a range of complex
cellular repair machinery. Our understanding of these DNA repair processes has advanced …

In CRISPR/Cas9 genome editing, the tight and persistent target binding of Cas9 provides an
opportunity for efficient genetic and epigenetic modification on genome. In particular …

Base editors (BEs) are genome editing agents that install point mutations with high efficiency
and specificity. Due to their reliance on uracil and inosine DNA damage intermediates …

Lessons learned from decades-long practice in the transplantation of hematopoietic stem
and progenitor cells (HSPCs) to treat severe inherited disorders or cancer, have set the …

As a versatile genome editing tool, the CRISPR-Cas9 system induces DNA double-strand
breaks at targeted sites to activate mainly two DNA repair pathways: HDR which allows …

Gene knock-in can be defined as the introduction of precisely determined modifications,
insertions, or replacements to the genome, which enables the generation of reporter cells …

Breast cancer, a prevalent disease with significant mortality rates, often presents treatment
challenges due to its complex genetic makeup. This review explores the potential of …

Retrons are promising gene editing tools because they can produce multi-copy single-
stranded DNA in cells via self-primed reverse transcription. However, their potential for …