Next-generation sequencing: insights to advance clinical investigations of the microbiome

CR Wensel, JL Pluznick, SL Salzberg… - The Journal of clinical …, 2022 - Am Soc Clin Investig
Next-generation sequencing (NGS) technology has advanced our understanding of the
human microbiome by allowing for the discovery and characterization of unculturable …

Applications of machine learning in human microbiome studies: a review on feature selection, biomarker identification, disease prediction and treatment

LJ Marcos-Zambrano… - Frontiers in …, 2021 - frontiersin.org
The number of microbiome-related studies has notably increased the availability of data on
human microbiome composition and function. These studies provide the essential material …

MicrobiomeAnalyst 2.0: comprehensive statistical, functional and integrative analysis of microbiome data

Y Lu, G Zhou, J Ewald, Z Pang, T Shiri… - Nucleic Acids …, 2023 - academic.oup.com
Microbiome studies have become routine in biomedical, agricultural and environmental
sciences with diverse aims, including diversity profiling, functional characterization, and …

Microbiome definition re-visited: old concepts and new challenges

G Berg, D Rybakova, D Fischer, T Cernava… - Microbiome, 2020 - Springer
The field of microbiome research has evolved rapidly over the past few decades and has
become a topic of great scientific and public interest. As a result of this rapid growth in …

Comparison between 16S rRNA and shotgun sequencing data for the taxonomic characterization of the gut microbiota

F Durazzi, C Sala, G Castellani, G Manfreda… - Scientific reports, 2021 - nature.com
In this paper we compared taxonomic results obtained by metataxonomics (16S rRNA gene
sequencing) and metagenomics (whole shotgun metagenomic sequencing) to investigate …

[HTML][HTML] Developmental trajectory of the healthy human gut microbiota during the first 5 years of life

J Roswall, LM Olsson, P Kovatcheva-Datchary… - Cell host & …, 2021 - cell.com
The gut is inhabited by a densely populated ecosystem, the gut microbiota, that is
established at birth. However, the succession by which different bacteria are incorporated …

Using MicrobiomeAnalyst for comprehensive statistical, functional, and meta-analysis of microbiome data

J Chong, P Liu, G Zhou, J Xia - Nature protocols, 2020 - nature.com
MicrobiomeAnalyst is an easy-to-use, web-based platform for comprehensive analysis of
common data outputs generated from current microbiome studies. It enables researchers …

Standardized multi-omics of Earth's microbiomes reveals microbial and metabolite diversity

JP Shaffer, LF Nothias, LR Thompson, JG Sanders… - Nature …, 2022 - nature.com
Despite advances in sequencing, lack of standardization makes comparisons across studies
challenging and hampers insights into the structure and function of microbial communities …

Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis

JS Johnson, DJ Spakowicz, BY Hong… - Nature …, 2019 - nature.com
The 16S rRNA gene has been a mainstay of sequence-based bacterial analysis for
decades. However, high-throughput sequencing of the full gene has only recently become a …

Unambiguous identification of fungi: where do we stand and how accurate and precise is fungal DNA barcoding?

R Lücking, MC Aime, B Robbertse, AN Miller… - IMA fungus, 2020 - Springer
True fungi (Fungi) and fungus-like organisms (eg Mycetozoa, Oomycota) constitute the
second largest group of organisms based on global richness estimates, with around 3 …