Cardiomyocyte maturation: advances in knowledge and implications for regenerative medicine

E Karbassi, A Fenix, S Marchiano, N Muraoka… - Nature Reviews …, 2020 - nature.com
Our knowledge of pluripotent stem cell (PSC) biology has advanced to the point where we
now can generate most cells of the human body in the laboratory. PSC-derived …

Cardiomyocyte maturation: new phase in development

Y Guo, WT Pu - Circulation research, 2020 - Am Heart Assoc
Maturation is the last phase of heart development that prepares the organ for strong,
efficient, and persistent pumping throughout the mammal's lifespan. This process is …

Human-iPSC-derived cardiac stromal cells enhance maturation in 3D cardiac microtissues and reveal non-cardiomyocyte contributions to heart disease

E Giacomelli, V Meraviglia, G Campostrini, A Cochrane… - Cell stem cell, 2020 - cell.com
Cardiomyocytes (CMs) from human induced pluripotent stem cells (hiPSCs) are functionally
immature, but this is improved by incorporation into engineered tissues or forced contraction …

In situ expansion, differentiation, and electromechanical coupling of human cardiac muscle in a 3D bioprinted, chambered organoid

ME Kupfer, WH Lin, V Ravikumar, K Qiu… - Circulation …, 2020 - Am Heart Assoc
Rationale: One goal of cardiac tissue engineering is the generation of a living, human pump
in vitro that could replace animal models and eventually serve as an in vivo therapeutic …

Functional screening in human cardiac organoids reveals a metabolic mechanism for cardiomyocyte cell cycle arrest

RJ Mills, DM Titmarsh, X Koenig… - Proceedings of the …, 2017 - National Acad Sciences
The mammalian heart undergoes maturation during postnatal life to meet the increased
functional requirements of an adult. However, the key drivers of this process remain poorly …

Metabolically driven maturation of human-induced-pluripotent-stem-cell-derived cardiac microtissues on microfluidic chips

N Huebsch, B Charrez, G Neiman, B Siemons… - Nature biomedical …, 2022 - nature.com
The immature physiology of cardiomyocytes derived from human induced pluripotent stem
cells (hiPSCs) limits their utility for drug screening and disease modelling. Here we show …

Single-cell resolution of temporal gene expression during heart development

DM DeLaughter, AG Bick, H Wakimoto, D McKean… - Developmental cell, 2016 - cell.com
Activation of complex molecular programs in specific cell lineages governs mammalian
heart development, from a primordial linear tube to a four-chamber organ. To characterize …

Drug screening in human PSC-cardiac organoids identifies pro-proliferative compounds acting via the mevalonate pathway

RJ Mills, BL Parker, GA Quaife-Ryan, HK Voges… - Cell stem cell, 2019 - cell.com
We have previously developed a high-throughput bioengineered human cardiac organoid
(hCO) platform, which provides functional contractile tissue with biological properties similar …

Stem cell therapy in heart diseases–cell types, mechanisms and improvement strategies

P Müller, H Lemcke, R David - Cellular Physiology and Biochemistry, 2018 - karger.com
A large number of clinical trials have shown stem cell therapy to be a promising therapeutic
approach for the treatment of cardiovascular diseases. Since the first transplantation into …

Single-cell transcriptomic analysis of cardiac differentiation from human PSCs reveals HOPX-dependent cardiomyocyte maturation

CE Friedman, Q Nguyen, SW Lukowski, A Helfer… - Cell stem cell, 2018 - cell.com
Cardiac differentiation of human pluripotent stem cells (hPSCs) requires orchestration of
dynamic gene regulatory networks during stepwise fate transitions but often generates …