Single molecule force spectroscopy is a powerful method that uses the mechanical
properties of DNA to explore DNA interactions. Here we describe how DNA stretching …

Highlights•Studying biomolecular folding with optical tweezers grants high dynamic
range.•Combining optical tweezers with molecular dynamics simulation enables accurate …

SYBR Gold is a commonly used and particularly bright fluorescent DNA stain, however, its
chemical structure is unknown and its binding mode to DNA remains controversial. Here, we …

There is a wide range of applications for non-covalent DNA binding ligands, and
optimization of such interactions requires detailed understanding of the binding …

We used single DNA molecule stretching to investigate DNA intercalation by ethidium and
three ruthenium complexes. By measuring ligand-induced DNA elongation at different …

DNA-binding small molecules are widespread in the cell and heavily used in biological
applications. Here, we use magnetic tweezers, which control the force and torque applied to …

Precisely known ligand-induced conformation change and complex chemical labeling of the
DNA sequence with probe molecules are often needed for the signal generation in most of …

In this review we focus on the idea of establishing connections between the mechanical
properties of DNA–ligand complexes and the physical chemistry of DNA–ligand interactions …

Actinomycin D (ActD) is a small molecule with strong antibiotic and anticancer activity.
However, its biologically relevant DNA-binding mechanism has never been resolved, with …

The last decade has seen rapid development in single molecule manipulation of RNA and
DNA. Measuring the response force for a particular manipulation has allowed the free …