RNase T1 is the best known representative of a large family of ribonucleolytic proteins
secreted by fungi, mostly Aspergillus and Penicillium species. Ribotoxins stand out among …

Fungi establish a complex network of biological interactions with other organisms in nature.
In many cases, these involve the production of toxins for survival or colonization purposes …

A single-chain fusion protein that directed the cytolytic activity of α-sarcin to A33 tumor
antigen expressing cells was constructed and shown to effectively kill targeted cells …

Fungi produce various defense proteins against antagonists, including ribotoxins. These
toxins cleave a single phosphodiester bond within the universally conserved sarcin-ricin …

The mite fungal pathogen Hirsutella thompsonii produces a single polypeptide chain,
insecticidal protein named hirsutellin A (HtA) that is composed of 130 amino acid residues …

The fungal pathogen Hirsutella thompsonii produces an insecticidal protein named
hirsutellin A (HtA), which has been described to be toxic to several species of mites, insect …

Ribotoxins constitute a family of toxic extracellular fungal RNases that exert a highly specific
activity on a conserved region of the larger molecule of rRNA, known as the sarcin-ricin loop …

Hirsutellin (HtA) is intermediate in size between other ribotoxins and less specific microbial
RNases, and thus offers a unique chance to determine the minimal structural requirements …

Residue Tyr-48 in α-sarcin is conserved not only within the ribotoxin family, but also within
the larger group of extracellular fungal ribonucleases, best represented by RNase T1. A …

Restrictocin is a site-specific endoribonuclease that inactivates ribosomes by cleaving the
sarcin/ricin loop (SRL) of 23S− 28S rRNA. Here we present a kinetic and thermodynamic …