High protein concentration solutions are becoming increasingly important in the
pharmaceutical industry. The solution behavior of proteins at high concentrations can …

Four types of environmental effects that can affect macromolecular reactions in a living cell
are defined: nonspecific intermolecular interactions, side reactions, partitioning between …

Analytical ultracentrifugation remains pre-eminent among the methods used to study the
interactions of macromolecules under physiological conditions. Recent developments in …

The concentration-dependence of the diffusion and sedimentation coefficients (k D and ks,
respectively) of a protein can be used to determine the second virial coefficient (B 2), a …

Exact expressions for the static light scattering of a solution containing up to three species of
point-scattering solutes in highly nonideal solutions at arbitrary concentration are obtained …

A method for rapid detection and characterization of reversible associations of
macromolecules in solution is presented. A programmable dual-syringe infusion pump is …

Using an experimental technique recently developed in this laboratory (Fernández C. and
AP Minton. 2008. Anal. Biochem. 381: 254–257), the Rayleigh light scattering of solutions of …

A simple method is described for the calculation of two-and three-dimensional phase
diagrams describing stability and coexistence curves or surfaces separating one-and two …

Fluorescence correlation spectroscopy (FCS) was used to measure the translational
diffusion of labeled apomyoglobin (tracer) in concentrated solutions of ribonuclease A and …

Diffusion of molecules in the crowded and charged interior of the cell has long been of
interest for understanding cellular processes. Here, we introduce a model system of …