BACKGROUND Proteins are synthesized on ribosomes as linear chains of amino acids and
must fold into unique three-dimensional structures to fulfill their biological functions. Protein …

The general secretory (Sec) pathway comprises an essential, ubiquitous and universal
export machinery for most proteins that integrate into, or translocate through, the plasma …

Problems arising during translation of mRNAs lead to ribosome stalling and collisions that
trigger a series of quality control events. However, the global cellular response to ribosome …

Pancreatic ductal adenocarcinoma (PDAC) tumors have a nutrient-poor, desmoplastic, and
highly innervated tumor microenvironment. Although neurons can release stimulatory factors …

Translation is one of the fundamental processes of life. It comprises the assembly of
polypeptides whose amino acid sequence corresponds to the codon sequence of an …

Quantitative views of cellular functions require precise measures of rates of biomolecule
production, especially proteins—the direct effectors of biological processes. Here, we …

Ribosome profiling, which involves the deep sequencing of ribosome-protected mRNA
fragments, is a powerful tool for globally monitoring translation in vivo. The method has …

Ribosome profiling has emerged as a technique for measuring translation comprehensively
and quantitatively by deep sequencing of ribosome-protected mRNA fragments. By …

The translation of messenger RNA (mRNA) into protein and the folding of the resulting
protein into an active form are prerequisites for virtually every cellular process and represent …

Recent studies highlight the importance of translational control in determining protein
abundance, underscoring the value of measuring gene expression at the level of translation …