Abstract Background Quantitative PCR (qPCR) aims to measure the DNA or RNA
concentration in diagnostic and biological samples based on the quantification cycle (Cq) …

RNA silencing at the transcriptional and posttranscriptional levels regulates endogenous
gene expression, controls invading transposable elements (TEs), and protects the cell …

Background Argonaute (AGO) proteins bind to small-interfering (si) RNAs and micro (mi)
RNAs to target RNA silencing against viruses, transgenes and in regulation of mRNAs …

The polymerase chain reaction (PCR) has matured from a labour-and time-intensive, low
throughput qualitative gel-based technique to an easily automated, rapid, high throughput …

Background Quantitative polymerase chain reaction (QPCR) is a widely applied analytical
method for the accurate determination of transcript abundance. Primers for QPCR have …

Sustainable agriculture encourages practices that present low risks to the environment and
human health. To this end, zein (corn protein) can be used to develop nanocarrier systems …

Quantitative real-time PCR (qPCR) has become a gold standard for the quantification of
nucleic acids and microorganism abundances, in which plasmid DNA carrying the target …

Background As a major component of plant cell wall, lignin plays important roles in
mechanical support, water transport, and stress responses. As the main cause for the …

Background qPCR has established itself as the technique of choice for the quantification of
gene expression. Procedures for conducting qPCR have received significant attention; …

The association between an adverse early life environment and increased susceptibility to
later-life metabolic disorders such as obesity, type 2 diabetes and cardiovascular disease is …