Publisher Summary The yeast Saccharomyces cerevisiae is a very suitable organism for
genetic analysis and now its genome has been completely sequenced; deletion mutants for …

▪ Abstract Three lines of evidence have converged on a multiprotein Mediator complex as a
conserved interface between gene-specific regulatory proteins and the general transcription …

To better understand yeast ribosome synthesis, we developed techniques for the rapid
harvesting and analysis of metabolically labeled cultures. Modeling of the resulting kinetic …

Cdt1 is essential for loading Mcm2–7 proteins into prereplicative complexes (pre-RCs)
during replication licensing and has been found in organisms as diverse as fission yeast …

Glutaredoxins are members of a superfamily of thiol disulfide oxidoreductases involved in
maintaining the redox state of target proteins. In Saccharomyces cerevisiae, two …

The budding yeast histone H3 variant, Cse4, replaces conventional histone H3 in
centromeric chromatin and, together with centromere-specific DNA-binding factors, directs …

Mitochondria perform a central function in the biogenesis of cellular iron–sulphur (Fe/S)
proteins. It is unknown to date why this biosynthetic pathway is indispensable for life, the …

The ends of linear eukaryotic chromosomes are protected by telomeres, which serve to
ensure proper chromosome replication and to prevent spurious recombination at …

Cytosolic and nuclear iron-sulfur (Fe-S) proteins play key roles in processes such as
ribosome maturation, transcription and DNA repair-replication. For biosynthesis of their Fe-S …

Evolutionarily diverse eukaryotic cells share many conserved proteins of unknown function.
Some are essential for cell viability,, emphasising their importance for fundamental …