Making sense of astrocytic calcium signals—from acquisition to interpretation

A Semyanov, C Henneberger, A Agarwal - Nature Reviews …, 2020 - nature.com
Astrocytes functionally interact with neurons and with other brain cells. Although not
electrically excitable, astrocytes display a complex repertoire of intracellular Ca2+ signalling …

Genetically encoded fluorescent biosensors illuminate the spatiotemporal regulation of signaling networks

EC Greenwald, S Mehta, J Zhang - Chemical reviews, 2018 - ACS Publications
Cellular signaling networks are the foundation which determines the fate and function of
cells as they respond to various cues and stimuli. The discovery of fluorescent proteins over …

Distinct fission signatures predict mitochondrial degradation or biogenesis

T Kleele, T Rey, J Winter, S Zaganelli, D Mahecic… - Nature, 2021 - nature.com
Mitochondrial fission is a highly regulated process that, when disrupted, can alter
metabolism, proliferation and apoptosis,–. Dysregulation has been linked to …

The growing and glowing toolbox of fluorescent and photoactive proteins

EA Rodriguez, RE Campbell, JY Lin, MZ Lin… - Trends in biochemical …, 2017 - cell.com
Over the past 20 years, protein engineering has been extensively used to improve and
modify the fundamental properties of fluorescent proteins (FPs) with the goal of adapting …

ER-mitochondria tethering by PDZD8 regulates Ca2+ dynamics in mammalian neurons

Y Hirabayashi, SK Kwon, H Paek, WM Pernice… - Science, 2017 - science.org
Interfaces between organelles are emerging as critical platforms for many biological
responses in eukaryotic cells. In yeast, the ERMES complex is an endoplasmic reticulum …

Optogenetic tools for subcellular applications in neuroscience

BR Rost, F Schneider-Warme, D Schmitz, P Hegemann - Neuron, 2017 - cell.com
The ability to study cellular physiology using photosensitive, genetically encoded molecules
has profoundly transformed neuroscience. The modern optogenetic toolbox includes …

Ca2+ channels couple spiking to mitochondrial metabolism in substantia nigra dopaminergic neurons

E Zampese, DL Wokosin, P Gonzalez-Rodriguez… - Science …, 2022 - science.org
How do neurons match generation of adenosine triphosphate by mitochondria to the
bioenergetic demands of regenerative activity? Although the subject of speculation, this …

Calcium channel ITPR2 and mitochondria–ER contacts promote cellular senescence and aging

DV Ziegler, D Vindrieux, D Goehrig, S Jaber… - Nature …, 2021 - nature.com
Cellular senescence is induced by stresses and results in a stable proliferation arrest
accompanied by a pro-inflammatory secretome. Senescent cells accumulate during aging …

Non-canonical function of IRE1α determines mitochondria-associated endoplasmic reticulum composition to control calcium transfer and bioenergetics

A Carreras-Sureda, F Jana, H Urra, S Durand… - Nature cell …, 2019 - nature.com
Mitochondria-associated membranes (MAMs) are central microdomains that fine-tune
bioenergetics by the local transfer of calcium from the endoplasmic reticulum to the …

Contact-ID, a tool for profiling organelle contact sites, reveals regulatory proteins of mitochondrial-associated membrane formation

C Kwak, S Shin, JS Park, M Jung… - Proceedings of the …, 2020 - National Acad Sciences
The mitochondria-associated membrane (MAM) has emerged as a cellular signaling hub
regulating various cellular processes. However, its molecular components remain unclear …