Toxicity testing in the 21st century: progress in the past decade and future perspectives
D Krewski, ME Andersen, MG Tyshenko… - Archives of …, 2020 - Springer
Advances in the biological sciences have led to an ongoing paradigm shift in toxicity testing
based on expanded application of high-throughput in vitro screening and in silico methods …
based on expanded application of high-throughput in vitro screening and in silico methods …
Best practices for translational disease modeling using human iPSC-derived neurons
SJ Engle, L Blaha, RJ Kleiman - Neuron, 2018 - cell.com
From the beginning, induced pluripotent stem cell (iPSC) technology was touted as a path to
improve our understanding of disease biology and enable drug discovery. Advances in …
improve our understanding of disease biology and enable drug discovery. Advances in …
[HTML][HTML] Biology-inspired microphysiological systems to advance patient benefit and animal welfare in drug development
U Marx, T Akabane, TB Andersson, E Baker… - Altex, 2020 - ncbi.nlm.nih.gov
The first microfluidic microphysiological systems (MPS) entered the academic scene more
than 15 years ago and were considered an enabling technology to human in vitro (patho) …
than 15 years ago and were considered an enabling technology to human in vitro (patho) …
Fetal bovine serum (FBS): past–present–future
J Van der Valk, K Bieback, C Buta, B Cochrane… - ALTEX-Alternatives to …, 2018 - altex.org
The supplementation of culture medium with fetal bovine serum (FBS, also referred to as
“fetal calf serum”) is still common practice in cell culture applications. Due to a number of …
“fetal calf serum”) is still common practice in cell culture applications. Due to a number of …
Evidence-based guidelines for controlling pH in mammalian live-cell culture systems
J Michl, KC Park, P Swietach - Communications biology, 2019 - nature.com
A fundamental variable in culture medium is its pH, which must be controlled by an
appropriately formulated buffering regime, since biological processes are exquisitely …
appropriately formulated buffering regime, since biological processes are exquisitely …
[PDF][PDF] Guidance document on good cell and tissue culture practice 2.0 (GCCP 2.0)
Abstract Good Cell and Tissue Culture Practice (GCCP) 2.0 is an updated guidance
document from GCCP 1.0 (published by ECVAM in 2005), which was developed for practical …
document from GCCP 1.0 (published by ECVAM in 2005), which was developed for practical …
Standardisation needs for organ on chip devices
Organ on chip (OoC) devices represent the cutting edge of biotechnologies, combining
advanced cell and tissue culture with microengineering. OoC is accelerating innovation in …
advanced cell and tissue culture with microengineering. OoC is accelerating innovation in …
In Vitro Research Reproducibility: Keeping Up High Standards
C Hirsch, S Schildknecht - Frontiers in pharmacology, 2019 - frontiersin.org
Concern regarding the reproducibility of observations in life science research has emerged
in recent years, particularly in view of unfavorable experiences with preclinical in vivo …
in recent years, particularly in view of unfavorable experiences with preclinical in vivo …
[HTML][HTML] Recommendation on test readiness criteria for new approach methods (NAM) in toxicology: exemplified for developmental neurotoxicity (DNT)
A Bal-Price, HT Hogberg, KM Crofton, M Daneshian… - Altex, 2018 - ncbi.nlm.nih.gov
Multiple non-animal-based test methods have never been formally validated. In order to use
such new approach methods (NAMs) in a regulatory context, criteria to define their …
such new approach methods (NAMs) in a regulatory context, criteria to define their …
Establishment of an a priori protocol for the implementation and interpretation of an in‐vitro testing battery for the assessment of developmental neurotoxicity
S Masjosthusmann, J Blum, K Bartmann… - EFSA Supporting …, 2020 - Wiley Online Library
In this project we set up a human cell‐based DNTin vitro testing strategy that is based on test
methods with high readiness and data generated therefrom. The methods underwent afit‐for …
methods with high readiness and data generated therefrom. The methods underwent afit‐for …