Advances in the biological sciences have led to an ongoing paradigm shift in toxicity testing
based on expanded application of high-throughput in vitro screening and in silico methods …

From the beginning, induced pluripotent stem cell (iPSC) technology was touted as a path to
improve our understanding of disease biology and enable drug discovery. Advances in …

The first microfluidic microphysiological systems (MPS) entered the academic scene more
than 15 years ago and were considered an enabling technology to human in vitro (patho) …

The supplementation of culture medium with fetal bovine serum (FBS, also referred to as
“fetal calf serum”) is still common practice in cell culture applications. Due to a number of …

A fundamental variable in culture medium is its pH, which must be controlled by an
appropriately formulated buffering regime, since biological processes are exquisitely …

Abstract Good Cell and Tissue Culture Practice (GCCP) 2.0 is an updated guidance
document from GCCP 1.0 (published by ECVAM in 2005), which was developed for practical …

Organ on chip (OoC) devices represent the cutting edge of biotechnologies, combining
advanced cell and tissue culture with microengineering. OoC is accelerating innovation in …

Concern regarding the reproducibility of observations in life science research has emerged
in recent years, particularly in view of unfavorable experiences with preclinical in vivo …

Multiple non-animal-based test methods have never been formally validated. In order to use
such new approach methods (NAMs) in a regulatory context, criteria to define their …

In this project we set up a human cell‐based DNTin vitro testing strategy that is based on test
methods with high readiness and data generated therefrom. The methods underwent afit‐for …