Total internal reflection fluorescence microscopy in cell biology
D Axelrod - Traffic, 2001 - Wiley Online Library
Key events in cellular trafficking occur at the cell surface, and it is desirable to visualize
these events without interference from other regions deeper within. This review describes a …
these events without interference from other regions deeper within. This review describes a …
Total internal reflection fluorescence microscopy
D Axelrod - Methods in cell biology, 1989 - Elsevier
Publisher Summary Total internal reflection fluorescence (TIRF) provides a means to
selectively excite just those fluorophores in an aqueous environment that are very near a …
selectively excite just those fluorophores in an aqueous environment that are very near a …
A new wave of cellular imaging
D Toomre, J Bewersdorf - Annual review of cell and …, 2010 - annualreviews.org
Fluorescence imaging methods that push or break the diffraction limit of resolution
(approximately 200 nm) have grown explosively. These super-resolution nanoscopy …
(approximately 200 nm) have grown explosively. These super-resolution nanoscopy …
[1] Total internal reflection fluorescence microscopy in cell biology
D Axelrod - Methods in enzymology, 2003 - Elsevier
Publisher Summary This chapter describes the total internal reflection fluorescence (TIRF)
microscopy in cell biology. TIRF microscopy (also called “evanescent wave microscopy”) …
microscopy in cell biology. TIRF microscopy (also called “evanescent wave microscopy”) …
Total internal reflection fluorescence microscopy
D Axelrod - Methods in cell biology, 2008 - Elsevier
Total internal reflection fluorescence microscopy (TIRFM), also known as evanescent wave
microscopy, is used in a wide range of applications, particularly to view single molecules …
microscopy, is used in a wide range of applications, particularly to view single molecules …
Selective imaging of surface fluorescence with very high aperture microscope objectives
D Axelrod - Journal of biomedical optics, 2001 - spiedigitallibrary.org
Three approaches to selective surface fluorescence detection are described. All three of
them depend on the use of extremely high numerical aperture (NA) objectives now …
them depend on the use of extremely high numerical aperture (NA) objectives now …
Calibrating evanescent-wave penetration depths for biological TIRF microscopy
Roughly half of a cell's proteins are located at or near the plasma membrane. In this
restricted space, the cell senses its environment, signals to its neighbors, and exchanges …
restricted space, the cell senses its environment, signals to its neighbors, and exchanges …
Tracking chromaffin granules on their way through the actin cortex
Quantitative time-lapse evanescent-wave imaging of individual fluorescently labelled
chromaffin granules was used for kinetic analysis of granule trafficking through a∼ 300-nm …
chromaffin granules was used for kinetic analysis of granule trafficking through a∼ 300-nm …
Evanescent excitation and emission in fluorescence microscopy
D Axelrod - Biophysical journal, 2013 - cell.com
Evanescent light—light that does not propagate but instead decays in intensity over a
subwavelength distance—appears in both excitation (as in total internal reflection) and …
subwavelength distance—appears in both excitation (as in total internal reflection) and …
Molecular Contrast Optical Coherence Tomography: A Review¶
C Yang - Photochemistry and Photobiology, 2005 - Wiley Online Library
This article reviews the current state of research on the use of molecular contrast agents in
optical coherence tomography (OCT) imaging techniques. After a brief discussion of the …
optical coherence tomography (OCT) imaging techniques. After a brief discussion of the …