Key events in cellular trafficking occur at the cell surface, and it is desirable to visualize
these events without interference from other regions deeper within. This review describes a …

Publisher Summary Total internal reflection fluorescence (TIRF) provides a means to
selectively excite just those fluorophores in an aqueous environment that are very near a …

Fluorescence imaging methods that push or break the diffraction limit of resolution
(approximately 200 nm) have grown explosively. These super-resolution nanoscopy …

Publisher Summary This chapter describes the total internal reflection fluorescence (TIRF)
microscopy in cell biology. TIRF microscopy (also called “evanescent wave microscopy”) …

Total internal reflection fluorescence microscopy (TIRFM), also known as evanescent wave
microscopy, is used in a wide range of applications, particularly to view single molecules …

Three approaches to selective surface fluorescence detection are described. All three of
them depend on the use of extremely high numerical aperture (NA) objectives now …

Roughly half of a cell's proteins are located at or near the plasma membrane. In this
restricted space, the cell senses its environment, signals to its neighbors, and exchanges …

Quantitative time-lapse evanescent-wave imaging of individual fluorescently labelled
chromaffin granules was used for kinetic analysis of granule trafficking through a∼ 300-nm …

Evanescent light—light that does not propagate but instead decays in intensity over a
subwavelength distance—appears in both excitation (as in total internal reflection) and …

This article reviews the current state of research on the use of molecular contrast agents in
optical coherence tomography (OCT) imaging techniques. After a brief discussion of the …