Over the past 20 years, protein engineering has been extensively used to improve and
modify the fundamental properties of fluorescent proteins (FPs) with the goal of adapting …

Fluorescent tools have revolutionized our ability to probe biological dynamics, particularly at
the cellular level. Fluorescent sensors have been developed on several platforms, utilizing …

The low photostability of fluorescent proteins is a limiting factor in many applications of
fluorescence microscopy. Here we present StayGold, a green fluorescent protein (GFP) …

We report the evolution of mScarlet3, a cysteine-free monomeric red fluorescent protein with
fast and complete maturation, as well as record brightness, quantum yield (75%) and …

We report the rational engineering of a remarkably stable yellow fluorescent protein
(YFP),'hyperfolder YFP'(hfYFP), that withstands chaotropic conditions that denature most …

Although StayGold is a bright and highly photostable fluorescent protein, its propensity for
obligate dimer formation may hinder applications in molecular fusion and membrane …

Cells in complex organisms undergo frequent functional changes, but few methods allow
comprehensive longitudinal profiling of living cells. Here we introduce scission-accelerated …

Effective protein quality control (PQC), essential for cellular health, relies on spatial
sequestration of misfolded proteins into defined inclusions. Here we reveal the coordination …

The contrast and resolution of images obtained with optical microscopes can be improved
by deconvolution and computational fusion of multiple views of the same sample, but these …

Cells bend their plasma membranes into highly curved forms to interact with the local
environment, but how shape generation is regulated is not fully resolved. Here, we report a …