Subcellular localization of RNAs has gained attention in recent years as a prevalent
phenomenon that influences numerous cellular processes. This is also evident for the large …

Fine-tuning cellular physiology in response to intracellular and environmental cues requires
precise temporal and spatial control of gene expression. High-resolution imaging …

We introduce APEX-seq, a method for RNA sequencing based on direct proximity labeling of
RNA using the peroxidase enzyme APEX2. APEX-seq in nine distinct subcellular locales …

INTRODUCTION Obtaining multiplexed molecular readouts from large numbers of single
cells in situ is an important technological goal to facilitate scientific discoveries in basic and …

Local translation regulates the axonal proteome, playing an important role in neuronal
wiring and axon maintenance. How axonal mRNAs are localized to specific subcellular sites …

Synthetic DNA motors have great potential to mimic natural protein motors in cells but the
operation of synthetic DNA motors in living cells remains challenging and has not been …

Image-based approaches to single-cell transcriptomics, in which RNA species are identified
and counted in situ via imaging, have emerged as a powerful complement to single-cell …

Translation is the fundamental biological process converting mRNA information into
proteins. Single-molecule imaging in live cells has illuminated the dynamics of RNA …

INTRODUCTION The copy number and intracellular localization of RNA are important
regulators of gene expression. Measurement of these properties at the transcriptome scale …

Filamentous fungi constitute a large group of eukaryotic microorganisms that grow by
forming simple tube-like hyphae that are capable of differentiating into more-complex …