Creating a Genetic Toolbox for the Carbon-Fixing, Nitrogen-Fixing and Dehalogenating Bacterium Xanthobacter autotrophicus

AF Van Voorhis, RS Sherbo - ACS Synthetic Biology, 2024 - ACS Publications
Xanthobacter autotrophicus is a metabolically flexible microorganism with two key
features:(1) The organism has adapted to grow on a wide variety of carbon sources …

Heterologous expression and characterization of an unsaturated glucuronyl hydrolase from Alteromonas sp. A321

R Xiao, C Du, H Li, M Zhang, Y Wu, L Xing, K Bu… - International Journal of …, 2024 - Elsevier
Strong promoters and stable mRNAs are essential for the overproduction of heterologous
proteins in Bacillus subtilis. To improve the strength of natural promoters and ensure robust …

Utilizing 5′ UTR Engineering Enables Fine-Tuning of Multiple Genes within Operons to Balance Metabolic Flux in Bacillus subtilis

J You, Y Wang, K Wang, Y Du, X Zhang, X Zhang… - Biology, 2024 - mdpi.com
Simple Summary The synthesis of natural or non-natural products in microorganisms
frequently involves multiple genes or pathways. Enhancing and balancing the expression of …

Fine-Tuning Gene Expression in Bacteria by Synthetic Promoters

S Okay - Synthetic Promoters: Methods and Protocols, 2024 - Springer
Promoters are key genetic elements in the initiation and regulation of gene expression. A
limited number of natural promoters has been described for the control of gene expression …

SubtiToolKit-a bioengineering kit for Bacillus subtilis and Gram-positive bacteria.

J Caro Astorga, K Malci, T Ellis, P James… - bioRxiv, 2024 - biorxiv.org
Building DNA constructs of increasing complexity is key to synthetic biology. Golden Gate
methods led to the creation of cloning toolkits-collections of modular standardized DNA parts …