Affinity chromatography: A review of trends and developments over the past 50 years
EL Rodriguez, S Poddar, S Iftekhar, K Suh… - … of Chromatography B, 2020 - Elsevier
The field of affinity chromatography, which employs a biologically-related agent as the
stationary phase, has seen significant growth since the modern era of this method began in …
stationary phase, has seen significant growth since the modern era of this method began in …
High performance affinity chromatography and related separation methods for the analysis of biological and pharmaceutical agents
The last few decades have witnessed the development of many high-performance
separation methods that use biologically related binding agents. The combination of HPLC …
separation methods that use biologically related binding agents. The combination of HPLC …
Testosterone meets albumin–the molecular mechanism of sex hormone transport by serum albumins
Serum albumin is the most abundant protein in mammalian blood plasma and is responsible
for the transport of metals, drugs, and various metabolites, including hormones. We report …
for the transport of metals, drugs, and various metabolites, including hormones. We report …
Determination of binding constants by ultrafast affinity extraction: Theoretical and experimental studies of optimum conditions for analysis
S Iftekhar, M Rauhauser, BD Hage, DS Hage - Journal of Chromatography …, 2023 - Elsevier
Ultrafast affinity extraction (UAE) is a form of microscale affinity HPLC that can be employed
to quickly measure equilibrium constants for solute-binding agent interactions in solution …
to quickly measure equilibrium constants for solute-binding agent interactions in solution …
Analysis of stereoselective drug interactions with serum proteins by high-performance affinity chromatography: A historical perspective
Z Li, DS Hage - Journal of pharmaceutical and biomedical analysis, 2017 - Elsevier
The interactions of drugs with serum proteins are often stereoselective and can affect the
distribution, activity, toxicity and rate of excretion of these drugs in the body. A number of …
distribution, activity, toxicity and rate of excretion of these drugs in the body. A number of …
Characterization of drug binding with alpha1-acid glycoprotein in clinical samples using ultrafast affinity extraction
Many drugs bind to serum transport proteins, which can affect both drug distribution and
activity in the body. α 1-Acid glycoprotein (AGP) is a key transport protein for basic and …
activity in the body. α 1-Acid glycoprotein (AGP) is a key transport protein for basic and …
Immune checkpoint regulators: A new era toward promising cancer therapy
During the last century, our battle against cancer has been inaugurated upon three main
approaches; surgery, radiation and chemotherapy. The latest findings on the effectiveness of …
approaches; surgery, radiation and chemotherapy. The latest findings on the effectiveness of …
Kinetic analysis by affinity chromatography
Important information on chemical processes in living systems can be obtained by the rates
at which these biological interactions occur. This review will discuss several techniques …
at which these biological interactions occur. This review will discuss several techniques …
Analysis of the binding of warfarin to glyoxal-and methylglyoxal-modified human serum albumin by ultrafast affinity extraction
Ultrafast affinity extraction (UAE) and affinity microcolumns containing immobilized human
serum albumin (HSA) were employed to evaluate the effect of advanced stage glycation on …
serum albumin (HSA) were employed to evaluate the effect of advanced stage glycation on …
Chromatographic studies of drug interactions with alpha1-acid glycoprotein by ultrafast affinity extraction and peak profiling
Interactions with serum proteins such as alpha 1-acid glycoprotein (AGP) can have a
significant effect on the behavior and pharmacokinetics of drugs. Ultrafast affinity extraction …
significant effect on the behavior and pharmacokinetics of drugs. Ultrafast affinity extraction …