Super-resolution microscopy for structural cell biology

S Liu, P Hoess, J Ries - Annual review of biophysics, 2022 - annualreviews.org
Super-resolution microscopy techniques, and specifically single-molecule localization
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …

A review of super-resolution single-molecule localization microscopy cluster analysis and quantification methods

IM Khater, IR Nabi, G Hamarneh - Patterns, 2020 - cell.com
Single-molecule localization microscopy (SMLM) is a relatively new imaging modality,
winning the 2014 Nobel Prize in Chemistry, and considered as one of the key super …

Deep learning enables fast and dense single-molecule localization with high accuracy

A Speiser, LR Müller, P Hoess, U Matti, CJ Obara… - Nature …, 2021 - nature.com
Single-molecule localization microscopy (SMLM) has had remarkable success in imaging
cellular structures with nanometer resolution, but standard analysis algorithms require …

Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software

D Sage, TA Pham, H Babcock, T Lukes, T Pengo… - Nature …, 2019 - nature.com
With the widespread uptake of two-dimensional (2D) and three-dimensional (3D) single-
molecule localization microscopy (SMLM), a large set of different data analysis packages …

Field-dependent deep learning enables high-throughput whole-cell 3D super-resolution imaging

S Fu, W Shi, T Luo, Y He, L Zhou, J Yang, Z Yang… - Nature …, 2023 - nature.com
Single-molecule localization microscopy in a typical wide-field setup has been widely used
for investigating subcellular structures with super resolution; however, field-dependent …

Parameter-free image resolution estimation based on decorrelation analysis

A Descloux, KS Grußmayer, A Radenovic - Nature methods, 2019 - nature.com
Super-resolution microscopy opened diverse new avenues of research by overcoming the
resolution limit imposed by diffraction. Exploitation of the fluorescent emission of individual …

Nuclear pores as versatile reference standards for quantitative superresolution microscopy

JV Thevathasan, M Kahnwald, K Cieśliński, P Hoess… - Nature …, 2019 - nature.com
Quantitative fluorescence and superresolution microscopy are often limited by insufficient
data quality or artifacts. In this context, it is essential to have biologically relevant control …

Single extracellular VEsicle nanoscopy

A Saftics, S Abuelreich, E Romano… - Journal of …, 2023 - Wiley Online Library
Extracellular vesicles (EVs) and their cargo constitute novel biomarkers. EV subpopulations
have been defined not only by abundant tetraspanins (eg, CD9, CD63 and CD81) but also …

Precisely and accurately localizing single emitters in fluorescence microscopy

H Deschout, FC Zanacchi, M Mlodzianoski, A Diaspro… - Nature …, 2014 - nature.com
Methods based on single-molecule localization and photophysics have brought nanoscale
imaging with visible light into reach. This has enabled single-particle tracking applications …

Sphingolipids and lipid rafts: Novel concepts and methods of analysis

E Bieberich - Chemistry and physics of lipids, 2018 - Elsevier
About twenty years ago, the functional lipid raft model of the plasma membrane was
published. It took into account decades of research showing that cellular membranes are not …