[HTML][HTML] Genome engineering for crop improvement and future agriculture

C Gao - Cell, 2021 - cell.com
Feeding the ever-growing population is a major challenge, especially in light of rapidly
changing climate conditions. Genome editing is set to revolutionize plant breeding and …

CRISPR screens in plants: approaches, guidelines, and future prospects

C Gaillochet, W Develtere, TB Jacobs - The Plant Cell, 2021 - academic.oup.com
Clustered regularly interspaced short palindromic repeat (CRISPR)-associated systems
have revolutionized genome engineering by facilitating a wide range of targeted DNA …

Multi-Knock—a multi-targeted genome-scale CRISPR toolbox to overcome functional redundancy in plants

Y Hu, P Patra, O Pisanty, A Shafir, ZM Belew… - Nature plants, 2023 - nature.com
Plant genomes are characterized by large and complex gene families that often result in
similar and partially overlapping functions. This genetic redundancy severely hampers …

[HTML][HTML] Use of CRISPR/Cas9-based gene editing to simultaneously mutate multiple homologous genes required for pollen development and male fertility in maize

X Liu, S Zhang, Y Jiang, T Yan, C Fang, Q Hou, S Wu… - Cells, 2022 - mdpi.com
Male sterility represents an important trait for hybrid breeding and seed production in crops.
Although the genes required for male fertility have been widely studied and characterized in …

Accelerated domestication of new crops: Yield is key

G Luo, J Najafi, PMP Correia, MDL Trinh… - Plant and Cell …, 2022 - academic.oup.com
Sustainable agriculture in the future will depend on crops that are tolerant to biotic and
abiotic stresses, require minimal input of water and nutrients and can be cultivated with a …

Guidelines for optimized gene knockout using CRISPR/Cas9

CV Campenhout, P Cabochette, AC Veillard… - …, 2019 - Taylor & Francis
CRISPR/Cas9 technology has evolved as the most powerful approach to generate genetic
models both for fundamental and preclinical research. Despite its apparent simplicity, the …

Establishment of new convenient two-line system for hybrid production by targeting mutation of OPR3 in allopolyploid Brassica napus

H Cheng, M Hao, S Sang, Y Wen, Y Cai… - Horticulture …, 2023 - academic.oup.com
The two-line pollination control system, which usually depends on the utilization of
thermosensitive or photoperiod genic male-sterile lines, has been widely used in various …

CAFRIRice: CRISPR applicable functional redundancy inspector to accelerate functional genomics in rice

WJ Hong, YJ Kim, EJ Kim… - The Plant …, 2020 - Wiley Online Library
Rice (Oryza sativa L.) is a staple crop with agricultural traits that have been intensively
investigated. However, despite the variety of mutant population and multiomics data that …

ModelTeller: model selection for optimal phylogenetic reconstruction using machine learning

S Abadi, O Avram, S Rosset, T Pupko… - Molecular biology and …, 2020 - academic.oup.com
Statistical criteria have long been the standard for selecting the best model for phylogenetic
reconstruction and downstream statistical inference. Although model selection is regarded …

CRISPR/Cas system: applications and prospects for maize improvement

Y Jiang, K Sun, X An - ACS Agricultural Science & Technology, 2022 - ACS Publications
Maize (Zea mays) is one of the most important cereal crops to secure food supplies in the
world. A growing world population requires new approaches to facilitate and accelerate …