pIGLET: Safe harbor landing sites for reproducible and efficient transgenesis in zebrafish
RL Lalonde, HH Wells, CL Kemmler… - Science …, 2024 - science.org
Standard zebrafish transgenesis involves random transgene integration with resource-
intensive screening. While phiC31 integrase–based attP/attB recombination has streamlined …
intensive screening. While phiC31 integrase–based attP/attB recombination has streamlined …
Dermal appendage-dependent patterning of zebrafish atoh1a+ Merkel cells
TL Brown, EC Horton, EW Craig, CEA Goo, EC Black… - Elife, 2023 - elifesciences.org
Touch system function requires precise interactions between specialized skin cells and
somatosensory axons, as exemplified by the vertebrate mechanosensory Merkel cell-neurite …
somatosensory axons, as exemplified by the vertebrate mechanosensory Merkel cell-neurite …
Next-generation plasmids for transgenesis in zebrafish and beyond
CL Kemmler, HR Moran, BF Murray… - …, 2023 - journals.biologists.com
Transgenesis is an essential technique for any genetic model. Tol2-based transgenesis
paired with Gateway-compatible vector collections has transformed zebrafish transgenesis …
paired with Gateway-compatible vector collections has transformed zebrafish transgenesis …
Multiple embryonic sources converge to form the pectoral girdle skeleton in zebrafish
S Kuroda, RL Lalonde, TA Mansour… - Nature …, 2024 - nature.com
The morphological transformation of the pectoral/shoulder girdle is fundamental to the water-
to-land transition in vertebrate evolution. Although previous studies have resolved the …
to-land transition in vertebrate evolution. Although previous studies have resolved the …
Lineage labeling with zebrafish hand2 Cre and CreERT2 recombinase CRISPR knock-ins
Z Ming, F Liu, HR Moran, RL Lalonde, M Adams… - bioRxiv, 2024 - biorxiv.org
Background: The ability to generate endogenous Cre recombinase drivers using CRISPR–
Cas9 knock–in technology allows lineage tracing, cell type specific gene studies, and in vivo …
Cas9 knock–in technology allows lineage tracing, cell type specific gene studies, and in vivo …
[HTML][HTML] Zebrafish for the Study of Enhancer Activity in Human Traits and Disease
E Morice, C Calhoun, R Lalonde… - Zebrafish Research-An …, 2023 - intechopen.com
Enhancers are genetically encoded elements that are critical for controlling gene
expression. Despite the importance of enhancers in development, normal biological …
expression. Despite the importance of enhancers in development, normal biological …
Instantaneous visual genotyping and facile site-specific transgenesis via CRISPR-Cas9 and phiC31 integrase
J Ma, W Zhang, S Rahimialiabadi, NU Ganesh… - Biology …, 2024 - journals.biologists.com
ABSTRACT Here, we introduce 'TICIT', targeted integration by CRISPR-Cas9 and integrase
technologies, which utilizes the site-specific DNA recombinase–phiC31 integrase–to insert …
technologies, which utilizes the site-specific DNA recombinase–phiC31 integrase–to insert …
[PDF][PDF] Next-generation plasmids for transgenesis in zebrafish and beyond
AF Puller, K Ditrychová, S Bötschi, M Affolter, N Rohner… - edoc.unibas.ch
Transgenesis is an essential technique for any genetic model. Tol2-based transgenesis
paired with Gateway-compatible vector collections has transformed zebrafish transgenesis …
paired with Gateway-compatible vector collections has transformed zebrafish transgenesis …