Ionic atmosphere effect on the absorption spectrum of a flavoprotein: a reminder to consider solution ions
BD Dratch, Y Orozco-Gonzalez, G Gadda… - The Journal of …, 2021 - ACS Publications
This study utilizes the FMN-dependent NADH: quinone oxidoreductase from Pseudomonas
aeruginosa PAO1 to investigate the effect of introducing an active site negative charge on …
aeruginosa PAO1 to investigate the effect of introducing an active site negative charge on …
The 4-α-Glucanotransferase AcbQ Is Involved in Acarbose Modification in Actinoplanes sp. SE50/110
S Nölting, C März, L Jacob, M Persicke… - Microorganisms, 2023 - mdpi.com
The pseudo-tetrasaccharide acarbose, produced by Actinoplanes sp. SE50/110, is a α-
glucosidase inhibitor used for treatment of type 2 diabetes patients. In industrial production …
glucosidase inhibitor used for treatment of type 2 diabetes patients. In industrial production …
Kinetic solvent viscosity effects uncover an internal isomerization of the enzyme-substrate complex in Pseudomonas aeruginosa PAO1 NADH: quinone …
Abstract NAD (P) H: quinone oxidoreductases (NQOs) play an essential protective role as
antioxidants in the detoxification of quinones in both Prokaryotes and Eukaryotes. NQO from …
antioxidants in the detoxification of quinones in both Prokaryotes and Eukaryotes. NQO from …
Mutation of a distal gating residue modulates NADH binding in NADH: Quinone oxidoreductase from Pseudomonas aeruginosa PAO1
Enzymes require flexible regions to adopt multiple conformations during catalysis. The
mobile regions of enzymes include gates that modulate the passage of molecules in and out …
mobile regions of enzymes include gates that modulate the passage of molecules in and out …
Modifying Corynebacterium glutamicum by metabolic engineering for efficient synthesis of l-lysine
Z Liu, J Liu, F Zhang, W Zhang - Systems Microbiology and …, 2024 - Springer
This study described that a low-producing mutagenic strain was transformed to a l-lysine
high-producing recombinant strain by optimizing the l-lysine metabolic pathway of …
high-producing recombinant strain by optimizing the l-lysine metabolic pathway of …
On the use of noncompetitive kinetic isotope effects to investigate flavoenzyme mechanism
K Francis, G Gadda - Methods in Enzymology, 2019 - Elsevier
This account describes the application of kinetic isotope effects (KIEs) to investigate the
mechanistic properties of flavin dependent enzymes. Assays can be conducted during …
mechanistic properties of flavin dependent enzymes. Assays can be conducted during …
Mechanistic investigation of a non-catalytic gating residue in NADH: Quinone oxidoreductase from Pseudomonas aeruginosa PA01
BM Moni - 2022 - scholarworks.gsu.edu
NADH: quinone oxidoreductase from Pseudomonas aeruginosa PAO1 (NQO, PA1024, EC
1.6. 5.9) is a recently reclassified flavin-dependent enzyme that catalyzes the two-electron …
1.6. 5.9) is a recently reclassified flavin-dependent enzyme that catalyzes the two-electron …
Kinetic Investigation of a Presumed Nitronate Monooxygenase from Pseudomonas aeruginosa PAO1 Establishes a New Class of NAD(P)H:Quinone Reductases
PA0660 from Pseudomonas aeruginosa PAO1 is currently classified as a hypothetical
nitronate monooxygenase (NMO), but no evidence at the transcript or protein level has been …
nitronate monooxygenase (NMO), but no evidence at the transcript or protein level has been …
The Ionic Atmosphere Effect on the Absorption Spectrum of a Flavoprotein: A Reminder to Consider the Importance of Solution Ions
Ionizable residues and monoatomic ions in solution modulate enzyme catalysis and the
structural stability of proteins; however, the delicate interplay between these short-range …
structural stability of proteins; however, the delicate interplay between these short-range …
Functional Importance of Non-Catalytic Residues in the Active Site of NADH: Quinone Oxidoreductase From Pseudomonas aeruginosa PA01
B Dratch, G Gadda - 2021 - scholarworks.gsu.edu
NADH: quinone oxidoreductase (NQO) from Pseudomonas aeruginosa PA01 is an FMN-
dependent enzyme that utilizes NADH to catalyze the two-electron reduction of a wide …
dependent enzyme that utilizes NADH to catalyze the two-electron reduction of a wide …