Metabolism and DNA replication are the two most fundamental biological functions in life.
The catabolic branch of metabolism breaks down nutrients to produce energy and …

Structural and biochemical studies have revealed the basic principles of how the replisome
duplicates genomic DNA, but little is known about its dynamics during DNA replication. We …

ABSTRACT DNA repair is essential for cell survival. In all domains of life, error-prone and
error-free repair pathways ensure maintenance of genome integrity under stress. Mutagenic …

Abstract In Escherichia coli, the DnaB helicase forms the basis for the assembly of the DNA
replication complex. The stability of DnaB at the replication fork is likely important for …

Despite extensive studies on DNA replication, the exchange mechanisms of DNA
polymerase during replication remain unclear. Existing models propose that this exchange …

The glycolytic enzyme PykA has been reported to drive the metabolic control of replication
through a mechanism involving PykA moonlighting functions on the essential DnaE …

Most biological processes in living cells rely on interactions between proteins. Live-cell
compatible approaches that can quantify to what extent a given protein participates in homo …

Abstract 3D single-molecule tracking microscopy has enabled measurements of protein
diffusion in living cells, offering information about protein dynamics and cellular …

Replication forks must respond to changes in nutrient conditions, especially in bacterial
cells. By investigating the single-molecule dynamics of replicative helicase DnaC, DNA …

This chapter reviews the current knowledge on the organization and replication of bacterial
genomes with a special emphasis on the initiation of DNA replication. Most bacteria contain …