In this review we will focus on the current status and views concerning the production of
antibody fragments and antibody fusion proteins by yeasts and filamentous fungi. We will …

In this article, recent progress related to the use of different types of polypeptide fusion
handles or 'tags' for the purification of recombinant proteins are critically discussed. In …

The methylotrophic yeast Pichia pastoris has been used for the expression of many proteins,
including antibody fragments. However, limitations became obvious especially when …

Humanization of monoclonal antibodies by complementary determinant region (CDR)-
grafting has become a standard procedure to improve the clinical usage of animal …

A novel protein purification method was developed using microbial polyhydroxyalkanoates
(PHA) granule-associated protein phasin, a pH-inducible self-cleaving intein and PHA …

Catalytic antibodies made it feasible to develop new catalysts, which had previously been
the subject of research. Scientists have discovered natural antibodies that can hydrolyze …

Yarrowia lipolytica and Kluyveromyces lactis secretion vectors were constructed and
assessed for the expression of heterologous proteins. An anti-Ras single-chain antibody …

Horseradish peroxidase is a key enzyme in bio-and immunochemical analysis. New
approaches in functional expression of the peroxidase gene in E. coli cells and the …

Antibodies represent an interesting protein framework on which catalytic functions can be
grafted. In previous studies, we have reported the characterization of the catalytic antibody …

The first full length IgG produced in Pichia pastoris was reported in late 1980. However, use
of a wild-type Pichia expression system to produce IgGs with human-like N-linked glycans …