Transcriptional regulation is achieved through combinatorial interactions between regulatory
elements in the human genome and a vast range of factors that modulate the recruitment …

The transcription cycle can be roughly divided into three stages: initiation, elongation, and
termination. Understanding the molecular events that regulate all these stages requires a …

Transcription occurs in stochastic bursts. Early models based upon RNA hybridisation
studies suggest bursting dynamics arise from alternating inactive and permissive states …

Immunity relies on the heterogeneity of immune cells and their ability to respond to pathogen
challenges. In the adaptive immune system, lymphocytes display a highly diverse antigen …

Single-cell analysis has revealed that transcription is dynamic and stochastic, but tools are
lacking that can determine the mechanism operating at a single gene. Here we utilize single …

Background The advent of high throughput RNA-seq at the single-cell level has opened up
new opportunities to elucidate the heterogeneity of gene expression. One of the most …

Transcription of genes can be discontinuous, occurring in pulses or bursts. It is not clear how
properties of transcriptional pulses vary between different genes. We compared the pulsing …

Dictyostelium has a mature technology for molecular-genetic manipulation based around
transfection using several different selectable markers, marker re-cycling, homologous …

Diffusible signals are known to orchestrate patterning during embryogenesis, yet diffusion is
sensitive to noise. The fact that embryogenesis is remarkably robust suggests that additional …

Single-cell microscopy studies have the potential to provide an unprecedented view of gene
expression with exquisite spatial and temporal sensitivity. However, there is a challenge to …