The serine protease inhibitors (SPIs) are widely distributed in living organisms like bacteria,
fungi, plants, and humans. The main function of SPIs as protease enzymes is to regulate the …

Highlights•Molecular farming has become established in niche markets.•Mucosally
delivered products benefit from in planta bioencapsulation.•Synthetic biology/genome …

Highlights•Transient expression is an effective method for the co-expression of proteins.•A
number of vectors are available to facilitate this use.•They can be used to produce complex …

Recombinant proteins expressed in plants have been emerged as a novel branch of the
biopharmaceutical industry, offering practical and safety advantages over traditional …

Agroinfiltrated Nicotiana benthamiana is a flexible and scalable platform for recombinant
protein (RP) production, but its great potential is hampered by plant proteases that degrade …

Addressing new challenges in global health and biosecurity requires responsive and
accessible platforms for the manufacture of preventative or therapeutic interventions …

Although the field of molecular farming has significantly matured over the last years, some
obstacles still need to be resolved. A major limiting factor for a broader application of plant …

Recombinant proteins are of paramount importance for research, industrial and medical use.
Numerous expression chassis are available for recombinant protein production, and while …

The unfolded protein response (UPR) allows cells to cope with endoplasmic reticulum (ER)
stress induced by accumulation of misfolded proteins in the ER. Due to its sensitivity to …

During the last two decades, the production of pharmaceutical proteins in plants evolved
from proof of concept to established technology adopted by several biotechnological …