Protein transport through a narrow solid-state nanopore at high voltage: experiments and theory
We report experimentally the transport of an unfolded protein through a narrow solid-state
nanopore of 3 nm diameter as a function of applied voltage. The random coil polypeptide …
nanopore of 3 nm diameter as a function of applied voltage. The random coil polypeptide …
Nanopore analysis: An emerging technique for studying the folding and misfolding of proteins
CA Madampage, O Tavassoly, C Christensen… - Prion, 2012 - Taylor & Francis
Nanopore analysis is an emerging technique that enables the investigation of the
conformation of a single peptide or protein molecule. Briefly, a pore is inserted into a …
conformation of a single peptide or protein molecule. Briefly, a pore is inserted into a …
Detection of peptides with different charges and lengths by using the aerolysin nanopore
The aerolysin nanopore has a high sensitivity for molecular sensing, owing to its super
narrow diameter and highly charged lumen structure. Here, taking advantages of its high …
narrow diameter and highly charged lumen structure. Here, taking advantages of its high …
Focus on protein unfolding through nanopores
In this review, we focus only on the unfolding of proteins through nanopores. We introduce
the principle of electrical detection with nanopores and how this technique provides …
the principle of electrical detection with nanopores and how this technique provides …
Thermostable virus portal proteins as reprogrammable adapters for solid-state nanopore sensors
Nanopore-based sensors are advancing the sensitivity and selectivity of single-molecule
detection in molecular medicine and biotechnology. Current electrical sensing devices are …
detection in molecular medicine and biotechnology. Current electrical sensing devices are …
Dynamics and energy contributions for transport of unfolded pertactin through a protein nanopore
To evaluate the physical parameters governing translocation of an unfolded protein across a
lipid bilayer, we studied protein transport through aerolysin, a passive protein channel, at the …
lipid bilayer, we studied protein transport through aerolysin, a passive protein channel, at the …
Pulling Peptides across Nanochannels: Resolving Peptide Binding and Translocation through the Hetero-oligomeric Channel from Nocardia farcinica
We investigated translocation of cationic peptides through nanochannels derived from the
Gram-positive bacterium Nocardia farcinica at the single-molecule level. The two subunits …
Gram-positive bacterium Nocardia farcinica at the single-molecule level. The two subunits …
Cu (II) and dopamine bind to αsynuclein and cause large conformational changes
O Tavassoly, S Nokhrin, OY Dmitriev… - The FEBS …, 2014 - Wiley Online Library
αSynuclein (AS) is an intrinsically disordered protein that can misfold and aggregate to
form Lewy bodies in dopaminergic neurons, a classic hallmark of Parkinson's disease. The …
form Lewy bodies in dopaminergic neurons, a classic hallmark of Parkinson's disease. The …
[HTML][HTML] Methamphetamine binds to α-synuclein and causes a conformational change which can be detected by nanopore analysis
O Tavassoly, JS Lee - FEBS letters, 2012 - Elsevier
α-Synuclein is an intrinsically disordered protein of 140 amino acids which is abundant in
dopaminergic neurons. Misfolding and aggregation of α-synuclein leads to the formation of …
dopaminergic neurons. Misfolding and aggregation of α-synuclein leads to the formation of …
The use of nanopore analysis for discovering drugs which bind to α-synuclein for treatment of Parkinson's disease
A major feature of Parkinson's disease is the formation of Lewy bodies in dopaminergic
neurons which consist of misfolded α-synuclein. The binding of natural products to α …
neurons which consist of misfolded α-synuclein. The binding of natural products to α …