Microbiological applications of high-resolution melting analysis

SYC Tong, PM Giffard - Journal of clinical microbiology, 2012 - Am Soc Microbiol
High-resolution melting (HRM) analysis uses real-time PCR instrumentation to interrogate
DNA sequence variation and is a low-cost, single-step, closed-tube method. Here we …

[PDF][PDF] High Resolution Melting as a rapid, reliable, accurate and cost-effective emerging tool for genotyping pathogenic bacteria and enhancing molecular …

M Tamburro, G Ripabelli - Ann Ig, 2017 - seu-roma.it
Introduction. Rapid, reliable and accurate molecular typing methods are essential for
outbreaks detection and infectious diseases control, for monitoring the evolution and …

Emergence of Klebsiella pneumoniae ST307 Co-Producing CTX-M with SHV and KPC from Paediatric Patients at Shenzhen Children's Hospital, China

S Patil, H Chen, C Guo, X Zhang, PG Ren… - Infection and drug …, 2021 - Taylor & Francis
Aim We investigated the clonal diversity of carbapenemase-producing Klebsiella
pneumoniae isolates from the Shenzhen Children's Hospital, China, and drew conclusions …

Sequencing independent molecular typing of Staphylococcus aureus isolates: approach for infection control and clonal characterization

K Dufkova, M Bezdicek, K Cuprova… - Microbiology …, 2022 - Am Soc Microbiol
Staphylococcus aureus is a major bacterial human pathogen that causes a wide variety of
clinical manifestations. The main aim of the presented study was to determine and optimize …

Reduction of formaldehyde emission from urea-formaldehyde resin with a small quantity of graphene oxide

K Saito, Y Hirabayashi, S Yamanaka - RSC advances, 2021 - pubs.rsc.org
Graphene oxide (GO) has theoretically been identified as a candidate for adsorbing
formaldehyde molecules. However, whether GO can actually serve as a scavenger for …

Identification of highly variable sequence fragments in unmapped reads for rapid bacterial genotyping

M Nykrynova, V Barton, M Bezdicek, M Lengerova… - BMC genomics, 2022 - Springer
Background Bacterial genotyping is a crucial process in outbreak investigation and
epidemiological studies. Several typing methods such as pulsed-field gel electrophoresis …

Rapid high-resolution melting genotyping scheme for Escherichia coli based on MLST derived single nucleotide polymorphisms

M Bezdicek, M Nykrynova, K Sedlar, S Kralova… - Scientific reports, 2021 - nature.com
Routinely used typing methods including MLST, rep-PCR and whole genome sequencing
(WGS) are time-consuming, costly, and often low throughput. Here, we describe a novel mini …

EasyPrimer: user-friendly tool for pan-PCR/HRM primers design. Development of an HRM protocol on wzi gene for fast Klebsiella pneumoniae typing

M Perini, A Piazza, S Panelli, D Di Carlo, M Corbella… - Scientific reports, 2020 - nature.com
In this work we present EasyPrimer, a user-friendly online tool developed to assist pan-PCR
and High Resolution Melting (HRM) primer design. The tool finds the most suitable regions …

Identifying critically ill patients at risk for inappropriate antibiotic therapy: a pilot study of a point-of-care decision support alert

ST Micek, KM Heard, M Gowan, MH Kollef - Critical care medicine, 2014 - journals.lww.com
Objective: To develop an automated alert aimed at reducing inappropriate antibiotic therapy
of serious healthcare-associated infections. Design: Single-center cohort study from …

A new look at the drug-resistance investigation of uropathogenic E. coli strains

W Adamus-Białek, Ł Lechowicz… - Molecular Biology …, 2017 - Springer
Bacterial drug resistance and uropathogenic tract infections are among the most important
issues of current medicine. Uropathogenic Escherichia coli strains are the primary factor of …