[HTML][HTML] Wheat root systems as a breeding target for climate resilience

ES Ober, S Alahmad, J Cockram, C Forestan… - Theoretical and Applied …, 2021 - Springer
In the coming decades, larger genetic gains in yield will be necessary to meet projected
demand, and this must be achieved despite the destabilizing impacts of climate change on …

Targeted mutagenesis with sequence‐specific nucleases for accelerated improvement of polyploid crops: Progress, challenges, and prospects

D May, K Paldi, F Altpeter - The Plant Genome, 2023 - Wiley Online Library
Many of the world's most important crops are polyploid. The presence of more than two sets
of chromosomes within their nuclei and frequently aberrant reproductive biology in …

[HTML][HTML] Highly efficient generation of canker-resistant sweet orange enabled by an improved CRISPR/Cas9 system

X Huang, Y Wang, N Wang - Frontiers in Plant Science, 2022 - frontiersin.org
Sweet orange (Citrus sinensis) is the most economically important species for the citrus
industry. However, it is susceptible to many diseases including citrus bacterial canker …

[HTML][HTML] Multiallelic, targeted mutagenesis of magnesium chelatase with CRISPR/Cas9 provides a rapidly scorable phenotype in highly polyploid sugarcane

A Eid, C Mohan, S Sanchez, D Wang… - Frontiers in Genome …, 2021 - frontiersin.org
Genome editing with sequence-specific nucleases, such as clustered regularly interspaced
short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9), is revolutionizing …

[HTML][HTML] The heat is on: a simple method to increase genome editing efficiency in plants

J Blomme, W Develtere, A Köse, J Arraiza Ribera… - BMC Plant …, 2022 - Springer
Background Precision genome mutagenesis using CRISPR/Cas has become the standard
method to generate mutant plant lines. Several improvements have been made to increase …

Efficient gene activation in plants by the MoonTag programmable transcriptional activator

JA Casas-Mollano, MH Zinselmeier… - Nucleic acids …, 2023 - academic.oup.com
CRISPR/Cas-based transcriptional activators have been developed to induce gene
expression in eukaryotic and prokaryotic organisms. The main advantages of CRISPR/Cas …

[HTML][HTML] Strategies and methods for improving the efficiency of CRISPR/Cas9 gene editing in plant molecular breeding

J Zhou, X Luan, Y Liu, L Wang, J Wang, S Yang, S Liu… - Plants, 2023 - mdpi.com
Following recent developments and refinement, CRISPR-Cas9 gene-editing technology has
become increasingly mature and is being widely used for crop improvement. The application …

[HTML][HTML] Gene editing in tree and clonal crops: progress and challenges

GS Goralogia, TP Redick, SH Strauss - In Vitro Cellular & Developmental …, 2021 - Springer
Because of the limitations inherent in conventional breeding of trees and clonally
propagated crops, gene editing is of great interest. Dozens of published papers attest to the …

DNA-free CRISPR/Cas9 genome editing system for oil palm protoplasts using multiple ribonucleoproteins (RNPs) complexes

J Norfaezah, MYA Masani, MDPMAA Fizree… - Industrial Crops and …, 2024 - Elsevier
CRISPR/Cas9 is a powerful genome editing tool applicable to diverse plant species.
However, the application of CRISPR/Cas9-ribonucleoproteins (RNPs) in oil palm (Elaeis …

[HTML][HTML] Leveraging the sugarcane CRISPR/Cas9 technique for genetic improvement of non-cultivated grasses

C Li, MA Iqbal - Frontiers in Plant Science, 2024 - frontiersin.org
Under changing climatic scenarios, grassland conservation and development have become
imperative to impart functional sustainability to their ecosystem services. These goals could …