Bacterial lipases: an overview of production, purification and biochemical properties

R Gupta, N Gupta, P Rathi - Applied microbiology and biotechnology, 2004 - Springer
Lipases, triacylglycerol hydrolases, are an important group of biotechnologically relevant
enzymes and they find immense applications in food, dairy, detergent and pharmaceutical …

Catalytic properties and potential applications of Bacillus lipases

M Guncheva, D Zhiryakova - Journal of Molecular Catalysis B: Enzymatic, 2011 - Elsevier
Up to date more than 70 lipases from the Bacillus and Geobacillus genera have been
isolated, but for most of them only basic biochemical properties have been reported. In …

Production, purification, characterization, and applications of lipases

R Sharma, Y Chisti, UC Banerjee - Biotechnology advances, 2001 - Elsevier
Lipases (triacylglycerol acylhydrolases, EC 3.1. 1.3) catalyze the hydrolysis and the
synthesis of esters formed from glycerol and long-chain fatty acids. Lipases occur widely in …

Production, purification, and characterization of lipase from thermophilic and alkaliphilic Bacillus coagulans BTS-3

S Kumar, K Kikon, A Upadhyay, SS Kanwar… - Protein expression and …, 2005 - Elsevier
A thermophilic isolate Bacillus coagulans BTS-3 produced an extracellular alkaline lipase,
the production of which was substantially enhanced when the type of carbon source …

Alkaline lipase from a novel strain Burkholderia multivorans: Statistical medium optimization and production in a bioreactor

N Gupta, V Sahai, R Gupta - Process Biochemistry, 2007 - Elsevier
An alkaline lipase from Burkholderia multivorans was produced within 15h of growth in a
14L bioreactor. An overall 12-fold enhanced production (58UmL− 1 and 36Umg− 1 protein) …

Purification and properties of a novel extra-cellular thermotolerant metallolipase of Bacillus coagulans MTCC-6375 isolate

SS Kanwar, IA Ghazi, SS Chimni, GK Joshi… - Protein expression and …, 2006 - Elsevier
A novel extra-cellular lipase from Bacillus coagulans MTCC-6375 was purified 76.4-fold by
DEAE anion exchange and Octyl Sepharose chromatography. The purified enzyme was …

Methods for inhibition of residual lipase activity in colorimetric assay: A comparative study

SS Kanwar, RK Kaushal, A Jawed, R Gupta, SS Chimni - 2005 - nopr.niscpr.res.in
A comparative study of various treatments for inhibition of the residual activity of a lipase
(obtained from Bacillus coagulans MTCC-6375) in a colorimetric assay using p-nitrophenyl …

Gene cloning, overexpression and characterization of a novel organic solvent tolerant and thermostable lipase from Galactomyces geotrichum Y05

J Yan, J Yang, L Xu, Y Yan - Journal of Molecular Catalysis B: Enzymatic, 2007 - Elsevier
Although the lipase of Geotrichum candidum has been extensively reported, little attention
has been focused on molecular genetic and biochemical characterizations of Galactomyces …

Characterization of 1, 3-regiospecific lipases from new Pseudomonas and Bacillus isolates

GK Meghwanshi, L Agarwal, K Dutt… - Journal of Molecular …, 2006 - Elsevier
Lipase producing ability of 120 bacterial isolates was examined qualitatively, resulting in 32
lipase producers, which were further screened for 1, 3-regiospecificity. Three Bacillus (GK-8 …

Production of extracellular lipase by Bacillus megaterium AKG-1 in submerged fermentation

A Sekhon, N Dahiya, RP Tewari, GS Hoondal - 2006 - nopr.niscpr.res.in
Bacillus megaterium AKG-1, a soil isolate, has been found to produce thermostable lipase
during submerged fermentation (SF). Mannitol at a concentration of 0.2%(w/v) was the best …