Metabolic abnormalities lead to the dysfunction of metabolic pathways and metabolite
accumulation or deficiency which is well-recognized hallmarks of diseases. Metabolite …

Many research questions in fields such as personalized medicine, drug screens or systems
biology depend on obtaining consistent and quantitatively accurate proteomics data from …

Liquid chromatography (LC) coupled with data-independent acquisition (DIA) mass
spectrometry (MS) has been increasingly used in quantitative proteomics studies. Here, we …

The dia-PASEF technology uses ion mobility separation to reduce signal interferences and
increase sensitivity in proteomic experiments. Here we present a two-dimensional peak …

Small, soluble metabolites not only are essential intermediates in intracellular biochemical
processes, but can also influence neighbouring cells when released into the extracellular …

Data-independent acquisition modes isolate and concurrently fragment populations of
different precursors by cycling through segments of a predefined precursor m/z range …

MaxDIA is a software platform for analyzing data-independent acquisition (DIA) proteomics
data within the MaxQuant software environment. Using spectral libraries, MaxDIA achieves …

Missing values weaken the power of label-free quantitative proteomic experiments to
uncover true quantitative differences between biological samples or experimental …

Spermidine (SPD) delays age-related pathologies in various organisms. SPD
supplementation overcame the impaired immunotherapy against tumors in aged mice by …

This review provides a brief overview of the development of data‐independent acquisition
(DIA) mass spectrometry‐based proteomics and selected DIA data analysis tools. Various …