It has been almost 25 years since the first report of the gene encoding a high-affinity
phosphate transporter (PT), PHO84, in yeast. Since then, an increasing number of yeast …

Since its inception, the CRISPR/Cas9 technology has been widely utilized for the targeted
insertion of donor DNAs into mammalian genomes. A shortcoming with the earlier knock-in …

Genomic insertions, duplications and insertion/deletions (indels), which account for~ 14% of
human pathogenic mutations, cannot be accurately or efficiently corrected by current gene …

Circular RNAs (circRNAs) represent a class of widespread and diverse endogenous RNAs
that may regulate gene expression in eukaryotes. However, the regulation and function of …

Background Precise genome editing via homology-directed repair (HDR) after double-
stranded DNA (dsDNA) cleavage facilitates functional genomic research and holds promise …

Key message CRISPR/Cas9-based multiplexed editing of SlHyPRP1 resulted in precise
deletions of its functional motif (s), thereby resulting in salt stress-tolerant events in cultivated …

Mutations in the BRCA2 gene are associated with sporadic and familial cancer, cause
genomic instability and sensitize cancer cells to inhibition by the poly (ADP-ribose) …

Herpesviruses infect the majority of the human population and can cause significant
morbidity and mortality. Herpes simplex virus (HSV) type 1 causes cold sores and herpes …

CRISPR–Cas9 screens have been widely adopted to analyze coding-gene functions, but
high-throughput screening of non-coding elements using this method is more challenging …

As the most common subtype of Leber congenital amaurosis (LCA), LCA10 is a severe
retinal dystrophy caused by mutations in the CEP290 gene. The most frequent mutation …