Immobilised enzymes: science or art?

L Cao - Current opinion in chemical biology, 2005 - Elsevier
Enzyme immobilisation is experiencing an important transition. Combinatorial approaches
are increasingly applied in the design of robust immobilised enzymes by rational …

Immobilized invertase

SM Kotwal, V Shankar - Biotechnology advances, 2009 - Elsevier
Invertase is a commercially important enzyme used for the hydrolysis of sucrose. The
hydrolysis of sucrose yields an equimolar mixture of glucose and fructose, known as invert …

Enzymes immobilized on montmorillonite K 10: Effect of adsorption and grafting on the surface properties and the enzyme activity

S Gopinath, S Sugunan - Applied Clay Science, 2007 - Elsevier
The enzymes α-amylase, glucoamylase and invertase were immobilized onto acid activated
montmorillonite (K 10) via two independent techniques—adsorption and grafting using …

Covalent immobilisation of invertase onto a reactive film composed of 2-hydroxyethyl methacrylate and glycidyl methacrylate: properties and application in a …

G Bayramoğlu, S Akgöl, A Bulut, A Denizli… - Biochemical Engineering …, 2003 - Elsevier
Invertase was covalently immobilised on the poly (hydroxyethyl methacrylate-co-glycidyl
methacrylate)(poly (HEMA–GMA)) film. The invertase immobilisation capacity of the films …

Immobilization of Baker's yeast invertase in PVA–alginate matrix using innovative immobilization technique

A Idris, NAM Zain, MS Suhaimi - Process Biochemistry, 2008 - Elsevier
This paper presents an innovative technique of immobilizing Baker's yeast invertase using
polyvinyl alcohol (PVA)–alginate matrix. Previous immobilization technique was improved …

[图书][B] Affinity chromatography

S Magdeldin - 2012 - books.google.com
Most will agree that one major achievement in the bio-separation techniques is affinity
chromatography. This coined terminology covers a myriad of separation approaches that …

Polyacrylamide–gelatine carrier system used for invertase immobilization

E Emregul, S Sungur, U Akbulut - Food Chemistry, 2006 - Elsevier
Invertase was immobilized into polyacrylamide–gelatin carrier system by chemical cross-
linking with chromium (III) acetate, chromium (III) sulphate, and potassium chromium (III) …

Hydrolysis of sucrose by invertase immobilized on nylon-6 microbeads

L Amaya-Delgado, ME Hidalgo-Lara… - Food chemistry, 2006 - Elsevier
A commercial extracellular invertase (EC 3.2. 1.26) from Saccharomyces cerevisiae has
been inmobilized by covalent bonding on novel microbeads of nylon-6 using …

Development of conductometric biosensor array for simultaneous determination of maltose, lactose, sucrose and glucose

OO Soldatkin, VM Peshkova, OY Saiapina… - Talanta, 2013 - Elsevier
The aim of this work was to develop an array of biosensors for simultaneous determination
of four carbohydrates in solution. Several enzyme systems selective to lactose, maltose …

Pyrrolidinyl ligand motif-assisted bovine serum albumin molecularly imprinted polymers with high specificity

M Wang, J Zhou, G Zhang, Q Liu, Q Zhang - Journal of Colloid and Interface …, 2022 - Elsevier
Ideal binding ligands for anchoring proteins are essential for the design and assembly of
desirable molecularly imprinted polymers (MIPs). In this study, bovine serum albumin-MIPs …