Proteins adopt different higher-order structures (HOS) to enable their unique biological
functions. Understanding the complexities of protein higher-order structures and dynamics …

Abstract I. Introduction 184 II. Mass Spectrometry and Disulfide Bond Determination 185 A.
Peptide Mass Analysis 185 B. Peptide Mass Analysis for Determination of the Disulfides of …

(Arg, Lys, His), except for disulfide-containing molecules, such as lysozyme and bovine
albumin. However, reduction of disulfide linkages with 1, 4-dlthlothrelto)(Cleland's reagent) …

Tandem mass spectrometry can be used to solve a number of protein structural problems
that are not amenable to conventional methods for amino acid sequencing. Typical …

Disulphide bonds in human recombinant tissue inhibitor of metalloproteinases (TIMP) were
assigned by resolving proteolytic digests of TIMP on reverse-phase hplc and sequencing …

abstract Disulfide bridges play a crucial role in the folding and structural stabilization of
many important extracellular peptide and protein molecules, including hormones, enzymes …

Mass spectrometry (MS) has become an Indispensable tool for peptide and protein structure
analysis because of three unique capabMties that enable It to beused to solve structural …

Matrix-assisted laser desorption/ionization (MALDI) ion trap mass spectrometry is shown to
be a powerful tool for the elucidation of protein modifications. Low-energy covalent bonds …

A gene fragment encoding the extracellular domain of the human growth hormone (hGH)
receptor from liver was cloned into a plasmid under control of the Escherichia coli alkaline …

Nerve growth factor (NGF) is a member of the neurotrophin family. These growth factors
support neuronal survival and differentiation. Neurotrophins are synthesized as pre-pro …