Advances in ultrahigh-throughput screening for directed enzyme evolution
U Markel, KD Essani, V Besirlioglu, J Schiffels… - Chemical Society …, 2020 - pubs.rsc.org
Enzymes are versatile catalysts and their synthetic potential has been recognized for a long
time. In order to exploit their full potential, enzymes often need to be re-engineered or …
time. In order to exploit their full potential, enzymes often need to be re-engineered or …
Enzyme-based digital bioassay technology–key strategies and future perspectives
H Noji, Y Minagawa, H Ueno - Lab on a Chip, 2022 - pubs.rsc.org
Digital bioassays based on single-molecule enzyme reactions represent a new class of
bioanalytical methods that enable the highly sensitive detection of biomolecules in a …
bioanalytical methods that enable the highly sensitive detection of biomolecules in a …
Highly fluorescent N-doped carbon dots with two-photon emission for ultrasensitive detection of tumor marker and visual monitor anticancer drug loading and delivery
P Gong, L Sun, F Wang, X Liu, Z Yan, M Wang… - Chemical Engineering …, 2019 - Elsevier
Early diagnosis and therapy are practically important to effectively prevent cancer and
improve patients' life quality. Herein, a simple and sensitive two-photon imaging fluorescent …
improve patients' life quality. Herein, a simple and sensitive two-photon imaging fluorescent …
Catalytic enzymes are active matter
Using a microscopic theory to analyze experiments, we demonstrate that enzymes are active
matter. Superresolution fluorescence measurements—performed across four orders of …
matter. Superresolution fluorescence measurements—performed across four orders of …
Digital bioassays: theory, applications, and perspectives
The development of bioassays with high sensitivity has received significant attention.
Enormous efforts based on a variety of signal amplification techniques have been made for …
Enormous efforts based on a variety of signal amplification techniques have been made for …
High-throughput analysis and protein engineering using microcapillary arrays
We describe a multipurpose technology platform, termed μSCALE (microcapillary single-cell
analysis and laser extraction), that enables massively parallel, quantitative biochemical and …
analysis and laser extraction), that enables massively parallel, quantitative biochemical and …
Facile and ultrasensitive fluorescence sensor platform for tumor invasive biomaker β-glucuronidase detection and inhibitor evaluation with carbon quantum dots …
Early detection and diagnosis have great practical significances for the effective prevention
and treatment of cancer. In this study, we developed a novel, facile and ultra-sensitive …
and treatment of cancer. In this study, we developed a novel, facile and ultra-sensitive …
Single‐molecule analysis determines isozymes of human alkaline phosphatase in serum
Alkaline phosphatase (ALP) is an important biomarker, as high levels of ALP in blood can
indicate liver disease or bone disorders. However, current clinical blood tests only measure …
indicate liver disease or bone disorders. However, current clinical blood tests only measure …
On-Chip enrichment system for digital bioassay based on aqueous two-phase system
Y Minagawa, S Nakata, M Date, Y Ii, H Noji - ACS nano, 2022 - ACS Publications
We developed an on-chip enrichment method based on an aqueous two-phase system of
dextran/polyethylene glycol mix, DEX/PEG ATPS, for digital bioassay. Accordingly, we …
dextran/polyethylene glycol mix, DEX/PEG ATPS, for digital bioassay. Accordingly, we …
Large scale analysis of the mutational landscape in β-glucuronidase: A major player of mucopolysaccharidosis type VII
The lysosomal storage disorders are a group of 50 unique inherited diseases characterized
by unseemly lipid storage in lysosomes. These malfunctions arise due to genetic mutations …
by unseemly lipid storage in lysosomes. These malfunctions arise due to genetic mutations …