Strategies for improving antimicrobial peptide production

S Deo, KL Turton, T Kainth, A Kumar, HJ Wieden - Biotechnology advances, 2022 - Elsevier
Antimicrobial peptides (AMPs) found in a wide range of animal, insect, and plant species are
host defense peptides forming an integral part of their innate immunity. Although the exact …

Reliable protein production in a Pseudomonas fluorescens expression system

DM Retallack, H Jin, L Chew - Protein expression and purification, 2012 - Elsevier
A bottleneck to product development can be reliable expression of active target protein. A
wide array of recombinant proteins in development, including an ever growing number of …

Extension of human GCSF serum half-life by the fusion of albumin binding domain

FY Nikravesh, S Shirkhani, E Bayat, Y Talebkhan… - Scientific Reports, 2022 - nature.com
Granulocyte colony stimulating factor (GCSF) can decrease mortality of patients undergo
chemotherapy through increasing neutrophil counts. Many strategies have been developed …

Synthetic pro‐peptide design to enhance the secretion of heterologous proteins by Saccharomyces cerevisiae

JS Cho, HJ Oh, YE Jang, HJ Kim, A Kim… - …, 2022 - Wiley Online Library
Heterologous protein production in Saccharomyces cerevisiae is a useful and effective
strategy with many advantages, including the secretion of proteins that require …

A topological refactoring design strategy yields highly stable granulopoietic proteins

J Skokowa, B Hernandez Alvarez, M Coles… - Nature …, 2022 - nature.com
Protein therapeutics frequently face major challenges, including complicated production,
instability, poor solubility, and aggregation. De novo protein design can readily address …

Evaluation of a new wide pore core–shell material (Aeris™ WIDEPORE) and comparison with other existing stationary phases for the analysis of intact proteins

S Fekete, R Berky, J Fekete, JL Veuthey… - … of Chromatography A, 2012 - Elsevier
The separation of large biomolecules such as proteins or monoclonal antibodies (mAbs) by
RPLC can be drastically enhanced thanks to the use of columns packed with wide-pore …

Safety and Biodistribution Profile of Poly (styrenyl acetal trehalose) and Its Granulocyte Colony Stimulating Factor Conjugate

JH Ko, NL Forsythe, MB Gelb, KMM Messina… - …, 2022 - ACS Publications
Poly (styrenyl acetal trehalose)(pSAT), composed of trehalose side chains linked to a
polystyrene backbone via acetals, stabilizes a variety of proteins and enzymes against …

Current status, and the developments of hosts and expression systems for the production of recombinant human cytokines

PK Das, A Sahoo, VD Veeranki - Biotechnology Advances, 2022 - Elsevier
Cytokines consist of peptides, proteins and glycoproteins, which are biological signaling
molecules, and boost cell-cell communication in immune reactions to stimulate cellular …

[HTML][HTML] Efficient process development of recombinant human granulocyte colony-stimulating factor (rh-GCSF) production in Escherichia coli

V Babaeipour, S Khanchezar, MR Mofid… - Iranian biomedical …, 2015 - ncbi.nlm.nih.gov
Background: The protein hormone granulocyte colony-stimulating factor (GCSF) stimulates
the production of white blood cells and plays an important role in medical treatment of …

Soluble prokaryotic overexpression and purification of bioactive human granulocyte colony-stimulating factor by maltose binding protein and protein disulfide …

BH Do, HB Ryu, P Hoang, BK Koo, H Choe - PloS one, 2014 - journals.plos.org
Human granulocyte colony-stimulating factor (hGCSF), a neutrophil-promoting cytokine, is
an effective therapeutic agent for neutropenia patients who have undergone several cancer …