RNA interference (RNAi) is a natural cellular process that effects post-transcriptional gene
silencing in eukaryotic systems. Small interfering RNA (siRNA) molecules are the key …

Transfection of mammalian cell lines is a widely used technique that requires significant
optimization, including transfection method or product used, DNA vector, cell density, media …

In this study, we characterized the conventional physicochemical properties of the
complexes formed by plasmid DNA (pDNA) and cationic liposomes (CL) composed of egg …

Background RNA interference (RNAi) is a target-specific gene silencing method that can be
used to determine gene functions and investigate host–pathogen interactions, as well as …

Human marrow stromal cells (hMSCs) are an attractive source for autologous cell and gene
therapies. In this study, we developed a highly efficient transfection method for hMSCs …

Triple negative breast cancer (TNBC) is one of the deadliest types of cancer for women of
different age groups. Frequently this cancer does not respond to conservative treatment …

The transfection of human NTera2/D1 teratocarcinoma-derived cell line (or NT2 cells)
represents a promising strategy for the delivery of exogenous proteins or biological agents …

5-Aminolevulinate synthase (ALAS; EC 2.3. 1.37) catalyzes the first committed step of heme
biosynthesis in animals. The erythroid-specific ALAS isozyme (ALAS2) is negatively …

Abstract pIRES2–EGFP was employed and a non‐target shRNA expressing plasmid was
constructed to simulate overexpression and RNAi (RNA interference) experiments …

Background. Nek2 is a serine/threonine kinase localized to the centrosome. It promotes cell
cycle progression from G2 to M by inducing centrosome separation. Recent studies have …