[HTML][HTML] New insights into tissue culture plant-regeneration mechanisms

Y Long, Y Yang, G Pan, Y Shen - Frontiers in plant science, 2022 - frontiersin.org
Plant regeneration occurs when plants repair or replace damaged structures based on the
totipotency and pluripotency of their cells. Tissue culture is one of the most widely used …

Recent advances in crop transformation technologies

Z Chen, JM Debernardi, J Dubcovsky, A Gallavotti - Nature plants, 2022 - nature.com
Agriculture is experiencing a technological inflection point in its history, while also facing
unprecedented challenges posed by human population growth and global climate changes …

Pluripotency acquisition in the middle cell layer of callus is required for organ regeneration

N Zhai, L Xu - Nature Plants, 2021 - nature.com
In plant tissue culture, callus forms from detached explants in response to a high-auxin-to-
low-cytokinin ratio on callus-inducing medium. Callus is a group of pluripotent cells because …

Spatial transcriptomics reveals light-induced chlorenchyma cells involved in promoting shoot regeneration in tomato callus

X Song, P Guo, K Xia, M Wang, Y Liu… - Proceedings of the …, 2023 - National Acad Sciences
Callus is a reprogrammed cell mass involved in plant regeneration and gene transformation
in crop engineering. Pluripotent callus cells develop into fertile shoots through shoot …

Uncovering the transcriptional regulatory network involved in boosting wheat regeneration and transformation

X Liu, XM Bie, X Lin, M Li, H Wang, X Zhang, Y Yang… - Nature Plants, 2023 - nature.com
Genetic transformation is important for gene functional study and crop improvement.
However, it is less effective in wheat. Here we employed a multi-omic analysis strategy to …

[HTML][HTML] Overexpression of the Transcription Factor GROWTH-REGULATING FACTOR5 Improves Transformation of Dicot and Monocot Species

J Kong, S Martin-Ortigosa, J Finer, N Orchard… - Frontiers in plant …, 2020 - frontiersin.org
Successful regeneration of genetically modified plants from cell culture is highly dependent
on the species, genotype, and tissue-type being targeted for transformation. Studies in some …

Application of developmental regulators to improve in planta or in vitro transformation in plants

Z Lian, CD Nguyen, L Liu, G Wang… - Plant Biotechnology …, 2022 - Wiley Online Library
Plant genetic transformation is a crucial step for applying biotechnology such as genome
editing to basic and applied plant science research. Its success primarily relies on the …

Wound-inducible WUSCHEL-RELATED HOMEOBOX 13 is required for callus growth and organ reconnection

M Ikeuchi, A Iwase, T Ito, H Tanaka, DS Favero… - Plant …, 2022 - academic.oup.com
Highly efficient tissue repair is pivotal for surviving damage-associated stress. Plants
generate callus upon injury to heal wound sites, yet regulatory mechanisms of tissue repair …

[HTML][HTML] Dynamic chromatin state profiling reveals regulatory roles of auxin and cytokinin in shoot regeneration

LY Wu, GD Shang, FX Wang, J Gao, MC Wan, ZG Xu… - Developmental Cell, 2022 - cell.com
Shoot regeneration is mediated by the sequential action of two phytohormones, auxin and
cytokinin. However, the chromatin regulatory landscapes underlying this dynamic response …

Stochastic gene expression drives mesophyll protoplast regeneration

M Xu, Q Du, C Tian, Y Wang, Y Jiao - Science Advances, 2021 - science.org
Cell pluripotency is fundamental to biology. It has long been known that differentiated
somatic plant cells may reacquire pluripotency, but the underlying mechanism remains …