Chloroplast genetic engineering overcomes concerns of gene containment, low levels of
transgene expression, gene silencing, positional and pleiotropic effects or presence of …

Most common strategies for gene transfer in plants derive from the fact that Agrobacterium
tumefaciens contains Ti plasmids which facilitate transfer of T-DNA from the plasmid …

Using a partially purified replication complex from tobacco chloroplasts, replication origins
have been localized to minimal sequences of 82 (pKN8, positions 137 683–137 764) and …

Chloroplasts contain circular DNA molecules which are found in low copy number in
proplastids but are amplified to very high copy number in actively dividing leaf cells. A …

Sequences described as chloroplast DNA replication origins were analysed in vivo by
creating deletion and insertion mutants via plastid transformation in tobacco. Deletion of the …

An ATP‐dependent DNA hecase has been purified to near homogeneity from pea
chloroplasts. The enzyme is a homodimer of 68‐kDa subunits. The purified enzyme shows …

We have mapped the origin of DNA replication (ori B) downstream of the 23 S rRNA gene in
each copy of the inverted repeat (IR) of tobacco chloroplast DNA between positions 130,502 …

Using 5′ end-labeled nascent strands of tobacco chloroplast DNA (ctDNA) as a probe,
replication displacement loop (D-loop) regions were identified. The strongest hybridization …

Three strains of Entamoeba histolytica and one of E. moshkovskii were analysed: circular
DNA was visualized by electron microscopy. Circles of various sized were observed; more …

The cDNA encoding p43, a DNA binding protein from pea chloroplasts (ct) that binds to
cognate DNA polymerase and stimulates the polymerase activity, has been cloned and …