Quorum sensing is the process by which bacteria communicate with each other to regulate
the expression of certain genes in a cell density-dependent manner. One group of signalling …

Various peptides can be obtained through protein enzymatic hydrolysis. Immobilized metal
ion affinity chromatography (IMAC) is one of the methods which can be used to separate …

The analysis and detection of targeted peptides has received due attention in many fields of
proteomics research, such as discovery of biomarkers. In this regard, capillary …

Arginine/lysine methylation is an important post-translational modification (PTM) involved in
DNA repairing, transcriptional regulation, etc. Immunoaffinity enrichment is currently the …

The precise measurement of protein abundance levels in highly complex biological samples
such as plasma remains challenging. The wide range of protein concentrations impairs the …

Histidine phosphorylation (pHis), which plays a key role in signal transduction in bacteria
and lower eukaryotes, has been shown to be involved in tumorigenesis. Due to its chemical …

Chemical modification of biological materials is indispensable for enrichment of
phosphorylated peptides. In this work, we synthesized a biomimetic honeycombed affinity …

A challenge for shotgun proteomics is the identification of low abundance proteins, which is
always hampered owing to the extreme complexity of protein digests and highly dynamic …

赖氨酸(K) 已被广泛用于靶向赖氨酸的交联剂设计、 蛋白质复合物的结构解析以及蛋白质-
蛋白质相互作用等研究领域。 在基于液相色谱-串联质谱(LC-MS/MS) 联用技术的“鸟枪法” …

Shotgun (bottom-up) approach has been widely applied in large-scale proteomics studies.
The inherent shortages of shotgun approach lie in that the generated peptides often …