The current fossil fuel reserves are not sufficient to meet the increasing demand and very
soon will become exhausted. Pollution, global warming, and inflated oil prices have led the …

Highlights•Microfluidics offers unique opportunities for bioprocess development.•
Environmental conditions can be precisely controlled on single-cell level.•Single-cell …

The ability to manipulate small fluid droplets, colloidal particles and single cells with the
precision and parallelization of modern-day computer hardware has profound applications …

Heterogeneity or segregation of microbial populations has been the subject of much
research, but the real impact of this phenomenon on bioprocesses remains poorly …

We have developed hydrogel-based virtual microfluidics as a simple and robust alternative
to complex engineered microfluidic systems for the compartmentalization of nucleic acid …

Medium optimization is a crucial step during cell culture for biopharmaceutics and
regenerative medicine; however, this step remains challenging, as both media and cells are …

Highlights•Yeasts create agility in manufacturing due to high titers and small time
scales.•Knowledge of host biology enables 'fit-to-purpose'strains for specific products.•Pichia …

Cellular heterogeneity influences bioprocess performance in ways that until date are not
completely elucidated. In order to account for this phenomenon in the design and operation …

Single cell analysis has received increasing attention recently in both academia and clinics,
and there is an urgent need for effective upstream cell sample preparation. Two extremely …

In recent years there have been tremendous advances in the versatility of magnetic shuttle
technology using nano/micro-scale magnets for digital magnetophoresis. While the …