Phase separation of signaling molecules promotes T cell receptor signal transduction

X Su, JA Ditlev, E Hui, W Xing, S Banjade, J Okrut… - Science, 2016 - science.org
Activation of various cell surface receptors triggers the reorganization of downstream
signaling molecules into micrometer-or submicrometer-sized clusters. However, the …

Cytoskeletal control of B cell responses to antigens

P Tolar - Nature Reviews Immunology, 2017 - nature.com
The actin cytoskeleton is essential for cell mechanics and has increasingly been implicated
in the regulation of cell signalling. In B cells, the actin cytoskeleton is extensively coupled to …

On the role of phase separation in the biogenesis of membraneless compartments

A Musacchio - The EMBO journal, 2022 - embopress.org
Molecular mechanistic biology has ushered us into the world of life's building blocks,
revealing their interactions in macromolecular complexes and inspiring strategies for …

Stoichiometry controls activity of phase-separated clusters of actin signaling proteins

LB Case, X Zhang, JA Ditlev, MK Rosen - Science, 2019 - science.org
Biomolecular condensates concentrate macromolecules into foci without a surrounding
membrane. Many condensates appear to form through multivalent interactions that drive …

Phase transitions of multivalent proteins can promote clustering of membrane receptors

S Banjade, MK Rosen - elife, 2014 - elifesciences.org
Clustering of proteins into micrometer-sized structures at membranes is observed in many
signaling pathways. Most models of clustering are specific to particular systems, and …

An optimized optogenetic clustering tool for probing protein interaction and function

A Taslimi, JD Vrana, D Chen, S Borinskaya… - Nature …, 2014 - nature.com
The Arabidopsis photoreceptor cryptochrome 2 (CRY2) was previously used as an
optogenetic module, allowing spatiotemporal control of cellular processes with light. Here …

Principles of self-organization and load adaptation by the actin cytoskeleton during clathrin-mediated endocytosis

M Akamatsu, R Vasan, D Serwas, MA Ferrin… - Elife, 2020 - elifesciences.org
Force generation by actin assembly shapes cellular membranes. An experimentally
constrained multiscale model shows that a minimal branched actin network is sufficient to …

Conserved interdomain linker promotes phase separation of the multivalent adaptor protein Nck

S Banjade, Q Wu, A Mittal… - Proceedings of the …, 2015 - National Acad Sciences
The organization of membranes, the cytosol, and the nucleus of eukaryotic cells can be
controlled through phase separation of lipids, proteins, and nucleic acids. Collective …

Mathematical modeling of eukaryotic cell migration: insights beyond experiments

G Danuser, J Allard, A Mogilner - Annual review of cell and …, 2013 - annualreviews.org
A migrating cell is a molecular machine made of tens of thousands of short-lived and
interacting parts. Understanding migration means understanding the self-organization of …

[HTML][HTML] Drops and fibers—how biomolecular condensates and cytoskeletal filaments influence each other

T Wiegand, AA Hyman - Emerging topics in life sciences, 2020 - ncbi.nlm.nih.gov
The cellular cytoskeleton self-organizes by specific monomer–monomer interactions
resulting in the polymerization of filaments. While we have long thought about the role of …