Bleaching‐Resistant Super‐Resolution Fluorescence Microscopy

J Kwon, MS Elgawish, SH Shim - Advanced Science, 2022 - Wiley Online Library
Photobleaching is the permanent loss of fluorescence after extended exposure to light and
is a major limiting factor in super‐resolution microscopy (SRM) that restricts spatiotemporal …

Chemical analysis of single cells and organelles

K Hu, TDK Nguyen, S Rabasco, PE Oomen… - Analytical …, 2020 - ACS Publications
life science, clinical diagnostics, drug development, etc. Recent years have witnessed the
emergence of innovative analytical methods with high sensitivity and spatial-temporal …

Beyond DNA: new probes for PAINT super-resolution microscopy

MME Tholen, RP Tas, Y Wang… - Chemical …, 2023 - pubs.rsc.org
In the last decade, point accumulation for imaging in nanoscale topography (PAINT) has
emerged as a versatile tool for single-molecule localization microscopy (SMLM). Currently …

Transient fluorescence labeling: Low affinity—high benefits

MM Perfilov, AS Gavrikov, KA Lukyanov… - International Journal of …, 2021 - mdpi.com
Fluorescent labeling is an established method for visualizing cellular structures and
dynamics. The fundamental diffraction limit in image resolution was recently bypassed with …

Lipocalin Blc is a potential heme‐binding protein

NG Bozhanova, MW Calcutt, WN Beavers… - FEBS …, 2021 - Wiley Online Library
Lipocalins are a superfamily of functionally diverse proteins defined by a well‐conserved
tertiary structure despite variation in sequence. Lipocalins bind and transport small …

Computational redesign of a fluorogen activating protein with Rosetta

NG Bozhanova, JM Harp, BJ Bender… - PLoS Computational …, 2021 - journals.plos.org
The use of unnatural fluorogenic molecules widely expands the pallet of available
genetically encoded fluorescent imaging tools through the design of fluorogen activating …