Structural mechanisms for replicating DNA in eukaryotes
I Attali, MR Botchan, JM Berger - Annual review of biochemistry, 2021 - annualreviews.org
The faithful and timely copying of DNA by molecular machines known as replisomes
depends on a disparate suite of enzymes and scaffolding factors working together in a …
depends on a disparate suite of enzymes and scaffolding factors working together in a …
Embracing Heterogeneity: Challenging the Paradigm of Replisomes as Deterministic Machines
The paradigm of cellular systems as deterministic machines has long guided our
understanding of biology. Advancements in technology and methodology, however, have …
understanding of biology. Advancements in technology and methodology, however, have …
Structure of a human replisome shows the organisation and interactions of a DNA replication machine
ML Jones, Y Baris, MRG Taylor, JTP Yeeles - The EMBO journal, 2021 - embopress.org
The human replisome is an elaborate arrangement of molecular machines responsible for
accurate chromosome replication. At its heart is the CDC45‐MCM‐GINS (CMG) helicase …
accurate chromosome replication. At its heart is the CDC45‐MCM‐GINS (CMG) helicase …
[HTML][HTML] A conserved mechanism for regulating replisome disassembly in eukaryotes
Replisome disassembly is the final step of eukaryotic DNA replication and is triggered by
ubiquitylation of the CDC45–MCM–GINS (CMG) replicative helicase,–. Despite being driven …
ubiquitylation of the CDC45–MCM–GINS (CMG) replicative helicase,–. Despite being driven …
[HTML][HTML] Single-strand DNA breaks cause replisome disassembly
DNA damage impedes replication fork progression and threatens genome stability. Upon
encounter with most DNA adducts, the replicative CMG helicase (CDC45-MCM2-7-GINS) …
encounter with most DNA adducts, the replicative CMG helicase (CDC45-MCM2-7-GINS) …
[HTML][HTML] The interplay of RNA: DNA hybrid structure and G-quadruplexes determines the outcome of R-loop-replisome collisions
R-loops are a major source of genome instability associated with transcription-induced
replication stress. However, how R-loops inherently impact replication fork progression is …
replication stress. However, how R-loops inherently impact replication fork progression is …
CMG helicase disassembly is essential and driven by two pathways in budding yeast
C Polo Rivera, TD Deegan, KPM Labib - The EMBO Journal, 2024 - embopress.org
The CMG helicase is the stable core of the eukaryotic replisome and is ubiquitylated and
disassembled during DNA replication termination. Fungi and animals use different enzymes …
disassembled during DNA replication termination. Fungi and animals use different enzymes …
Metabolic clogging of mannose triggers dNTP loss and genomic instability in human cancer cells
Y Harada, Y Mizote, T Suzuki, A Hirayama, S Ikeda… - Elife, 2023 - elifesciences.org
Mannose has anticancer activity that inhibits cell proliferation and enhances the efficacy of
chemotherapy. How mannose exerts its anticancer activity, however, remains poorly …
chemotherapy. How mannose exerts its anticancer activity, however, remains poorly …
[HTML][HTML] PARP1-dependent DNA-protein crosslink repair
Z Fábián, ES Kakulidis, IA Hendriks… - Nature …, 2024 - nature.com
DNA-protein crosslinks (DPCs) are toxic lesions that inhibit DNA related processes. Post-
translational modifications (PTMs), including SUMOylation and ubiquitylation, play a central …
translational modifications (PTMs), including SUMOylation and ubiquitylation, play a central …
[HTML][HTML] Nucleotide binding halts diffusion of the eukaryotic replicative helicase during activation
The eukaryotic replicative helicase CMG centrally orchestrates the replisome and leads the
way at the front of replication forks. Understanding the motion of CMG on the DNA is …
way at the front of replication forks. Understanding the motion of CMG on the DNA is …