Background Biocatalytic production of L‐phosphinothricin (L‐PPT) is currently the most
promising method. In this work, we use an Escherichia coli strain coexpressing of D‐amino …

Excessive nitrogen (N) discharged in water is a major cause of eutrophication and other
severe environmental issues. Biological N removal via heterotrophic nitrification and aerobic …

Agricultural plantations in Indonesia and Malaysia yield substantial waste, necessitating
proper disposal to address environmental concerns. Yet, these wastes, rich in starch and …

Hydroxytryptophan (5-HTP), a precursor of the neurotransmitter serotonin in mammals, has
demonstrated efficacy in treating various diseases such as depression, fibromyalgia and …

A peptidase S9 prolyl oligopeptidase domain from Thermotoga petrophila RKU-1 T (TpS9)
was over-expressed as an active, soluble and hyperstable lipolytic enzyme in the mesophilic …

In vitro transcription (IVT) using T7 RNA polymerase (RNAP) is integral to RNA research, yet
producing this enzyme in E. coli presents challenges regarding endotoxins and animal …

Klebsiella oxytoca is a resident of the human gut. However, certain K. oxytoca toxigenic
strains exist that secrete the nonribosomal peptide tilivalline (TV) cytotoxin. TV is a …

Herein, a step-by-step protocol for simultaneous detection of 20 amino acids commonly
present in cell culture media is described. The protocol facilitates detection of both primary …

This study presents a comprehensive analysis of scientific literature published between
2019 and 2024, indexed in Web of Science and Scopus databases. The review focuses on …

Анотація В роботі відображено комплексний аналіз даних наукової літератури,
опублікованої в період між 2019 і 2024 роками, відображеної в виданнях, що …