[HTML][HTML] High-content CRISPR screening
CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …
interrogation of gene function in a wide range of applications and species. In pooled …
Synthetic lethality as an engine for cancer drug target discovery
A Huang, LA Garraway, A Ashworth… - Nature reviews Drug …, 2020 - nature.com
The first wave of genetically targeted therapies for cancer focused on drugging gene
products that are recurrently mutated in specific cancer types. However, mutational analysis …
products that are recurrently mutated in specific cancer types. However, mutational analysis …
Ferroptosis surveillance independent of GPX4 and differentially regulated by sex hormones
Ferroptosis, a cell death process driven by iron-dependent phospholipid peroxidation, has
been implicated in various diseases. There are two major surveillance mechanisms to …
been implicated in various diseases. There are two major surveillance mechanisms to …
Copper induces cell death by targeting lipoylated TCA cycle proteins
Copper is an essential cofactor for all organisms, and yet it becomes toxic if concentrations
exceed a threshold maintained by evolutionarily conserved homeostatic mechanisms. How …
exceed a threshold maintained by evolutionarily conserved homeostatic mechanisms. How …
CAR T cell killing requires the IFNγR pathway in solid but not liquid tumours
Chimeric antigen receptor (CAR) therapy has had a transformative effect on the treatment of
haematologic malignancies,,,,–, but it has shown limited efficacy against solid tumours. Solid …
haematologic malignancies,,,,–, but it has shown limited efficacy against solid tumours. Solid …
Tumor aerobic glycolysis confers immune evasion through modulating sensitivity to T cell-mediated bystander killing via TNF-α
L Wu, Y Jin, X Zhao, K Tang, Y Zhao, L Tong, X Yu… - Cell Metabolism, 2023 - cell.com
Metabolic reprogramming toward glycolysis is a hallmark of cancer malignancy. The
molecular mechanisms by which the tumor glycolysis pathway promotes immune evasion …
molecular mechanisms by which the tumor glycolysis pathway promotes immune evasion …
Genome-wide CRISPR screens of T cell exhaustion identify chromatin remodeling factors that limit T cell persistence
T cell exhaustion limits antitumor immunity, but the molecular determinants of this process
remain poorly understood. Using a chronic stimulation assay, we performed genome-wide …
remain poorly understood. Using a chronic stimulation assay, we performed genome-wide …
7-Dehydrocholesterol dictates ferroptosis sensitivity
Y Li, Q Ran, Q Duan, J Jin, Y Wang, L Yu, C Wang… - Nature, 2024 - nature.com
Ferroptosis, a form of regulated cell death that is driven by iron-dependent phospholipid
peroxidation, has been implicated in multiple diseases, including cancer,–, degenerative …
peroxidation, has been implicated in multiple diseases, including cancer,–, degenerative …
High-speed fluorescence image–enabled cell sorting
Fast and selective isolation of single cells with unique spatial and morphological traits
remains a technical challenge. Here, we address this by establishing high-speed image …
remains a technical challenge. Here, we address this by establishing high-speed image …
ZBP1-dependent inflammatory cell death, PANoptosis, and cytokine storm disrupt IFN therapeutic efficacy during coronavirus infection
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus responsible for
coronavirus disease 2019 (COVID-19), continues to cause substantial morbidity and …
coronavirus disease 2019 (COVID-19), continues to cause substantial morbidity and …